Daura, Xavier

Ferrer-Navarro, Mario

Gibert, Isidre

Huedo, P.

Martínez, P.

Mongiardini, E.

Planell, Raquel

Torrent, Gerard

Vila, J.

Repositorio Institucional de la Universidad de Córdoba

S2. Microbial Proteomics  Poster P18 128  COMPARATIVE PROTEOMIC ANALYSIS OF COLLECTION AND CLINICAL-ISOLATE STRAINS OF PSEUDOMONAS AERUGINOSA M. Ferrer-Navarro (1), R. Planell (1), E. Mongiardini (1), G. Torrent (1), P. Huedo (1), P. Martinez (1), J. Vila (2), I. Gibert (1), X. Daura (1). (1) Institut de Biotecnologia i de Biomedicina, (2) Hospital Clinic de Barcelona. Pseudomonas aeruginosa is an opportunistic pathogen that is a major cause of infection-related mortality among individuals with immuno-compromised systems. Fatality rates among patients infected with P. aeruginosa are higher than among those infected with any other opportunistic gram-negative bacterium, partly due to the incidence of multiresistant strains. Because of its relevance in hospital environments, there is a continuous effort to extend the knowledge on the biology of this human pathogen. It is well known that in vitro serial passage of microorganisms causes changes in physiology and virulence-factor production. Thus, the collection strain PAO1 of P. aeruginosa is expected to display important differences at the proteome level with respect to new clinical isolates. Here, we present a comparative proteome analysis of a new clinical multiresistant isolate present in several hospitals in the area of Barcelona and the collection strain PAO1. In order to perform this comparative analysis we have used the DIGE technology coupled with protein identification by mass spectrometry. The identification of differentially expressed proteins in these strains shall provide a basis for the interpretation of their phenotypic differences at a biomolecular level, with a special focus on infection and resistance mechanisms. Acknowledgement: AntiPathoGN is supported by funding under the Seventh Research Framework Programme of the European Union (ref. HEALTH-F3-2009-223101). 

Comparative proteomic analysis of collection and clinical-isolate strains of psudomonas aeruginosa

Comparative proteomic analysis of collection and clinical-isolate strains of psudomonas aeruginosa

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Repositorio Institucional de la Universidad de Córdoba