Ariza-Mateos, David

Arroyo, M.

Jorrín-Novo, Jesús V.

Mallofret Carrera, E.

Marchal Gallardo, F.

Navarro Cerrillo, Rafael M.

Redondo-López, I.

Valero, J.

Repositorio Institucional de la Universidad de Córdoba

Poster  S2. Microbial Proteomics P37 147  ANALYSIS OF THE HOLM OAK (QUERCUS ILEX SUBSP. BALLOTA) POLLEN PROTEOME BY USING NLC-MS/MS   J. Valero (1), I. Redondo López (1), R.M. Navarro Cerrillo (2), D. Ariza Mateos (2), M. Arroyo (3), F. Marchal Gallardo (3), E. Mallofret Carrera (3), J.V. Jorrín Novo (1). (1) Dpt. of Biochemistry and Molecular Biology, University of Córdoba, Spain, (2) Department of Forestry Engineering, ETSIAM, University of Córdoba, Córdoba, Spain, (3) EGMASA. División de Sostenibilidad y Biodiversidad. Recolección y Certificación del Material Forestal de Reproducción, Spain. Our groups are carrying out a research project aimed at studying natural variability in Holm oak (Quercus ilex subsp. ballota (Desf.) Samp.), with some results already published (Jorge et al. 2005, 2006, Echevarría et al. 2009, Valero et al. 2010). The high variability found in the leaf 2-DE protein profile, even within the same tree and depending, among other factors, of the leaf orientation, position, sampling time, developmental stage, and environment, encouraged us to use other organs such as fruit and pollen for such as purpose, as they are suposed to have a less variable proteome. Biodiversity in plant species by using proteomics and simple experimental material such as pollen have a number of advantages if compared with other approaches and tissues. In the present work, a gel-free/label-free approach has been used to characterize the holm oak pollen proteome.  Proteins were extracted from pollen using the TCA-acetone-phenol protocol (Wang et al. 2006), as reported in Maldonado et al. (2008). Total protein extracts were digested with trypsin, tryptic peptides were separated by nano-HPLC and analyzed in an Orbitrap mass spectrometer. This analysis has allowed the identification of 529 protein species belonging to several functional categories, being the proteome dominated by enzymes of different pathways, protein fate, cell structure, disease, and defense. However, as typical for orphan tree species (Jorrín et al. 2009) and as experimented before in our previous studies on Q.ilex (Jorge et al. 2005, 2006, Echevarría-Zomeño et al. 2009) the absence of Quercus spp. DNA, proteins or ESTs entries in databases made the protein identification difficult as well, even when they have been properly extracted, separated and detected. This work is part of research projects financed by Spanish Ministry of Science and Innovation, their being FEDER cofinanced: CGL2008-04503-C03-01/BOS, AGL2002-00530, and AGL2009-12243-C02-02. 

Analysis of the holm oak (Quercu Ilex subs ballota) pollen proteome by using NLC-MS/MS

Analysis of the holm oak (Quercu Ilex subs ballota) pollen proteome by using NLC-MS/MS

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Repositorio Institucional de la Universidad de Córdoba