II Encuentro sobre nanociencia y nanotecnología de investigadores y tecnólogos de la Universidad de Córdoba. NANOUC

Camacho Delgado, Luis

Domínguez Vera, J.M.

Giner-Casares, Juan J.

Gálvez, N.

Martín-Romero, M.T.

Roldán-Carmona, C.

Watt, Richard K.

Repositorio Institucional de la Universidad de Córdoba

MIXED MONOLAYERS OF EICOSYLAMINE AND A BACTERIAL-FERRITIN PREPARED BY ADSORPTION TECHNIQUE AT THE AIR-WATER INTERFACE  C. Roldán-Carmona1, J.J Giner-Casares1, N. Gálvez2, J. M. Domínguez Vera2, Richard K. Watt3, M. T. Martín-Romero1,* and Luis Camacho1  1 Dpto. de Química Física y Termodinámica Aplicada, Universidad de Córdoba, Campus Universitario de Rabanales, C3 (Edificio Marie Curie), 2ª planta, 14014 Córdoba, (Spain). 2 Dpto. Química Inorgánica, Facultad de Ciencias, Universidad de Granada, 18071 (Spain). 3 Dept. of Chemistry and Biochemistry, Brigham Young University, 84602 Provo, UT (USA). q22rocac@uco.es  Natural ferritin is the iron storage protein of animals, plants and bacteria. This globular protein, with a diameter of ca. 12 nm, may hold approximately 5000 iron atoms. Because of its excellent magnetic properties, it is interesting as a potential component on nanotechnological devices. Several studies have been performed to obtain thin organized films containing ferritin molecules. Langmuir and Langmuir-Blodgett techniques have been revealed as very useful strategies to prepare such thin films. In previous works, mixed monolayers of ferritin attached to a mixed lipid matrix of eicosylamine (EA) and methyl stearate (SME) have been built by adsorption method [1,2]. In this work we have investigated a new type of ferritin, a bacterial-ferritin that contains porphyrinic groups covalently linked to its core, which properties are not only interesting for the magnetic but also the spectroscopic features. The adsorption properties of the bacterial-ferritin to a Langmuir monolayer of EA have been studied. The EA monolayer was spread on a different ferritin solutions as an aqueous subphase. The adsorption of the bacterial-ferritin at the air-water interface has been probed through a noticeable expansion on the surface pressure (π) – molecular area isotherms with respect to the EA monolayer on pure water, also Brewster angle microscopy (BAM), UV-vis reflection spectroscopy (ΔR) at the air water interface, as well as surface potential (ΔV) – molecular area measurements have been used for its characterization. The results show not only the retention of the bacterial-ferritin to the lipid matrix but also a more homogeneous and reproducible mixed film in comparison with similar systems [2].  Figura 1. π-A isotherms of EA monolayers formed on aqueous subphases containing different concentrations of bacterial-ferritin (in mg/mL). As an example, BAM images of a mixed film are shown.  References  [1] D. W. Britt, D. Möbius, V. Hlady, Phys. Chem. Chem. Phys. 2, 4594, (2000). [2] M. Clemente-León, E. Coronado, A. Soriano-Portillo, E. Colacio, J. M. Dominguez-Vera, N. Gálvez, R. Madueño, M. T. Martín-Romero, Langmuir 22, 6993, (2006). Area per EA molecule, Å220 40 60 80 100Surface Pressure, mN/m05101520253035EA5·10-310-22,4·10-30 mN/m21 mN/mP-20 

Mixed monolayers of eicosylamine and a bacterial-ferritin prepared by adsorption technique at the air-water interface

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