II Encuentro sobre nanociencia y nanotecnología de investigadores y tecnólogos de la Universidad de Córdoba. NANOUC

Fernández-Romero, J.M.

Gómez-Hens, A.

Sierra-Rodero, Marina

Repositorio Institucional de la Universidad de Córdoba

KINETIC STUDY OF THE INTERACTION OF GOLD NANOPARTICLES WITH THIOL COMPOUNDS:  DETERMINATION OF N-ACETYLCYSTEINE USING LIGHT SCATTERING DETECTION    M. Sierra-Rodero, J.M. Fernández-Romero and A. Gómez-Hens  Department of Analytical Chemistry, Marie Curie Annex Building, Campus of Rabanales, University of Córdoba, E-14071-Córdoba, Spain. qa1gohea@uco.es, http://www.uco.es/investiga/grupos/FQM-303     The aggregation of gold nanoparticles in the presence of several thiol compounds and the cationic surfactant cetyltrimethyl ammonium bromide has been kinetically studied using light scattering detection. This study has given rise to a simple and rapid method for the determination of N-acetylcysteine (NAC). The aggregation process has been monitored by measuring the initial reaction-rate (v0) and the light scattering signal at a prefixed-time (ΔIF10), using stopped-flow mixing technique, which makes the method applicable to automate routine analysis. Each measurement was obtained in about 15 s, using an integration time of 0.2 s. The dynamic range of the calibration graph obtained for NAC, using the initial rate method, was 2.9 - 60 µmol L-1, and the detection limit was 0.87 µmol L-1. Similar results were obtained for other thiol compounds assayed. The precision of the method, expressed as relative standard deviation, ranged between 0.6 and 3.5 %. The method was applied to the determination of NAC in several pharmaceutical samples with acceptable recoveries in all instances, which ranged between 97.7 and 101.1 %. P-30 

Kinetic study of the interaction of gold nanoparticles with thiol compounds: determination of n-acetylcysteine using light scattering detection

Abstract

Similar works

Full text

Available Versions

Repositorio Institucional de la Universidad de Córdoba