Medicinal plants a gift of nature are being used against various infections and diseases in the subcontinent since past history. Herbs were use as food ( vegetables ) and flavors for hundred of years in many parts of world. Whereas number of herbs have also been traditionally regarded as natural remedies for common ailments of human population. Furthermore some herbal plants are considered as house of medicines and played an important role in nearly every culture on earth, including Asia, Africa, Europe and the Americas. A study was conducted to analyze the Leaves and seeds of Foeniculum vulgare for different bioactive compounds those includes saponins , total proteins, amino acids, fat and flavonoids with one and two dimensional thin layer and column chromatography followed by spectrophotometric analysis. Results indicates that leaves contained higher concentration of flavonoids and fat. Where as level of Saponins, proteins, amino acids, total minerals and other organic compounds was high in seeds. The analysis of leave and seeds of Foeniculum vulgare for these valuable organic compounds will provide important raw materials that can be used for preparation of medicines in Pharmaceuticals companies. Furthermore interest of people in plant made medicines are increasing due to their concern about the side effects of powerful synthetic drugs and high prices of these medicines. It is expected that raw material obtained from plants will not only reduce the prices of medicines in the pharmaceutical market but also provide rapid and reliable system of treatment of various infection and disease of human populatio