Phytochemical constituents, functional group identification and antimicrobial activity of ethanolic extract of Solanum nigrum were investigated. The phytochemical screening of the crude extract revealed the presence of alkaloids, reducing sugars, tannis, flavonoids, Phlobatannis, and steriods. The extract were subjected for identification of functional groups using infra red spectrophotometer, presence of C=O, C-H, C=C and CO were identified, these bonding structures are responsible for presence of alkyl groups, methyl groups, alcohols, ethers, esters, carboxylic acid and anhydrides. And the crude ethanolic extract of Solanum nigrum were assayed for in vitro antimicrobial activity against gram positive and gram negative bacteria and they were also subjected for antifungal activity and the zone of inhibition were compared with control drug Penicillin and Nystatin. Staphylococcus aureus as the most sensitive strains exhibited maximum zone of inhibition about 25.7mm among gram positive organism tested. Escherichia coli exhibits 30.1mm zone than that of standard penicillin positive control. Trichophyton mentagrophytes exhibits maximum zone of inhibition of about 26.1mm among the tested fungal species
