Calteau, Alexandra

Kristiansen, Rikke

Le, Vang

McIlroy, Simon Jon

Nguyen, Hien Thi Thu

Nielsen, Jeppe Lund

Nielsen, Kåre Lehmann

Nielsen, Per Halkjær

Petrovski, Steve

Saunders, Aaron Marc

Seviour, Robert

Wimmer, Reinhard

English

    Aalborg UniversitetMetabolic model for Tetrasphaera involved in enhanced biological phosphorusremoval plantsKristiansen, Rikke; Nguyen, Hien Thi Thu; Saunders, Aaron Marc; Nielsen, Jeppe Lund;Wimmer, Reinhard; Le, Vang; Petrovski, Steve; McIlroy, Simon Jon; Seviour, Robert;Calteau, Alexandra; Nielsen, Kåre Lehmann; Nielsen, Per HalkjærPublication date:2012Document VersionEarly version, also known as pre-printLink to publication from Aalborg UniversityCitation for published version (APA):Kristiansen, R., Nguyen, H. T. T., Saunders, A. M., Nielsen, J. L., Wimmer, R., Le, V., Petrovski, S., McIlroy, S.J., Seviour, R., Calteau, A., Nielsen, K. L., & Nielsen, P. H. (2012). Metabolic model for Tetrasphaera involved inenhanced biological phosphorus removal plants. Poster presented at 7th Danish Conference on Biotechonologyand Molecular Biology, Vejle, Denmark.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: December 25, 20201 1 1 1 1 1 1 1,2 2 1 2 3 rkr@bio.aau.dk 1 2 3 INTRODUCTION Enhanced biological phosphorus removal (EBPR) is an environmentally friendly and cost-effective phosphorus removal process for wastewater. It is believed to be carried out mainly by certain polyphosphate accumulating organisms (PAOs) called Candidatus Accumulibacter. Recently, actinobacteria belonging to the genus Tetrasphaera have been identified as putative polyphosphate accumulating organisms. New investigations show that they comprise a larger fraction  of the biomass in full-scale Danish wastewater treatment plants than Candidatus Accumulibacter (8-12% and 3-7%, respectively) and may therefore be more important in the phosphorus removal process. Little is known about the diversity and physiology of Tetrasphaera but preliminary results indicate that their ecological niche is different from Candidatus Accumulibacter. AIM • Determine the diversity and abundance of Tetrasphaera in Danish treatment plants with enhanced biological phosphorus removal (EBPR). • Develop a metabolic model for phosphate-removing Tetrasphaera based on genomic investigations and pure culture studies. METHODS Identification and quantification Quantitative FISH using published and novel species-specific probes   Development of metabolic model • Paired-end genome sequencing of four Tetrasphaera isolates using the Illumina platform. • Annotation using MaGe (Microbial Genome Annotation Platform) from MicroScope. • Validating the genomic findings with pure culture studies. Fig. 2: FISH images of Tetrasphaera.  Probe Cluster Treatment Plant (% EUBmix ± SD) Ejby Mølle Hjørring Åbenrå Skive Odense Elo1250 1 0 0 < 1 3.2± 1.3 1.2 ± 1.0 Tet823 1 < 1 3.4 ±1.6 0 0 0 Tet266 1 5.5 ± 2.0 4.2±1.2 4.1±1.4 < 1 4.3 ± 1.0 Tet842 2 < 1 1.1 ± 1.0 3 ± 1.3 < 1 < 1 Tet831 2 0 0 3.1 ±1.5 0 0 Tet892 2 5.4 ± 1.3 4.4 ± 1.0 4.5 ± 1.5 4.3 ± 1.0 4.4 ± 1.0 Tet87 2 2.3 ± 1.7 1.2 ± 1 < 1 < 1 < 1 Tet174 2 5.2 ± 1.3 4 ± 1.0 2.9 ± 1.0 1.7 ± 1.0 4.5 ± 1.2 Tet654 3 6.8 ± 2.0 5.7 ± 1.1 5.5 ± 1.8 5.4 ± 2.0 5.2 ± 1.4 Tet19 3 4.3 ± 1.5 4.1 ± 1.0 3.2 ± 1.0 3.5 ± 1.0 4.3 ± 1.0 PAO mix (Accumulibacter) 5.4 ± 1.0 3.1 ± 1.3 4.8 ± 1.5 4.5 ± 1.0 5.4 ± 1.2 Table 1: Abundance of Tetrasphaera in Danish EBPR plants determined using qFISH. Fig. 1: Phylogenetic tree of 16S rRNA gene sequences of  Tetrasphaera from EBPR plants. The sequences group in 3 clusters. 2 clusters include isolated Tetrasphaera.  CONCLUSIONS • A high abundance and diversity of Tetrasphaera were identified in Danish full-scale EBPR plants. • Tetrasphaera are potentially novel and ecologically relevant polyphosphate accumulating organisms in full-scale EBPR plants with a different physiology in EBPR plants compared to Candidatus Accumulibacter. Identification and quantification Development of metabolic model Fig. 3: Simplified metabolic model of Candidatus Accumulibacter during the EBPR process (Martín et al., 2006). Pathway Accumulibacter T. australiensis T. elongata T. jenkinsii T. japonica Glycolysis + + + + + Gluconeogenesis + + + + + Glycogen synthesis + + + + + Glycogenolysis + + + + + TCA cycle + + + + + Glyoxylate shunt + - - - - PHA synthesis + - - - + Polyphosphate metabolism + + + + + Fermentation - + + + + Acetate uptake + + + + + Glucose uptake - + + + + Glutamate/aspartate uptake - + + + + Table 2: Potential metabolic pathways identified by genomic investigations.  +: present -: not present Fig. 5: Metabolic model for Tetrasphaera. The key metabolic features enabling Tetrasphaera to compete in full-scale EBPR plants are shown. 00.020.040.060.080.10.1200.20.40.60.811.20 2 4 6Concentration [mM] Glycogen Concentration [mM] Glucose or Phosphate Time [h] Anaerobic Aerobic Fig. 4: The ability of T. elongata to release Pi (triangles) and produce glycogen (diamonds) in the anaerobic phase feed with glucose (squares) (0-3 h) and take up Pi and consume glycogen in the aerobic phase (3.5-6.5 h).  

Metabolic model for Tetrasphaera involved in enhanced biological phosphorus removal plants

https://vbn.aau.dk/ws/files/74322356/Poster_Rikke_Munkebjerg.pdf

Metabolic model for Tetrasphaera involved in enhanced biological phosphorus removal plants

Abstract

Similar works

Full text

Available Versions

VBN