Fink, Trine

Frøbert, O.

Kastrup, J.

Rasmussen, Jeppe Grøndahl

Simonsen, U.

Stem cell therapy following acute myocardial infarction:comparative studies with adipose tissue-derived stem cells and bone marrow-derived stem cells

Frøbert, Ole

Kastrup, Jens

Simonsen, Ulf

    Aalborg UniversitetStem cell therapy following acute myocardial infarctionComparative studies with adipose tissue-derived stem cells and bone marrow-derived stemcellsRasmussen, Jeppe Grøndahl; Frøbert, Ole; Kastrup, Jens; Fink, Trine; Simonsen, UlfPublication date:2009Document VersionPublisher's PDF, also known as Version of recordLink to publication from Aalborg UniversityCitation for published version (APA):Rasmussen, J. G., Frøbert, O., Kastrup, J., Fink, T., & Simonsen, U. (2009). Stem cell therapy following acutemyocardial infarction: Comparative studies with adipose tissue-derived stem cells and bone marrow-derivedstem cells. Abstract from Danish Cardiovascular Research Academy, Summer Meeting, Sandbjerg, Denmark.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            ? You may not further distribute the material or use it for any profit-making activity or commercial gain            ? You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us at vbn@aub.aau.dk providing details, and we will remove access tothe work immediately and investigate your claim.Downloaded from vbn.aau.dk on: November 29, 2020Abstract 22  Stem Cell therapy following acute myocardial infarction. Comparative studies with Adipose Tissue-Derived Stem Cells and Bone Marrow-Derived Stem Cells Jeppe Grøndahl Rasmussen1, Ole Frøbert1,2, Jens Kastrup3, Trine Fink4,  and Ulf Simonsen1 1Institute of Pharmacology, Aarhus University;  2Department of Cardiology, Örebro University Hospital; 3Department of Cardiology, The Heart Centre, Rigshospitalet;  4Laboratory for Stem Cell Research, Aalborg University E-mail: jgra@farm.au.dk  Background The myocardium has a poor ability to regenerate after myocardial infarction. Studies have shown effect of stem cell transplantation (mainly bone marrow-derived stem cells) after myocardial infarction, with paracrine activity and neovascularisation playing an important role.  Adipose tissue-derived stem cells have been shown to be able to differentiate in an endothelial direction and have shown effect in stem cell treatments. This may depend on the fact that adipose tissue-derived stem cells, like bone marrow-derived stem cells, are of embryonal mesodermal origin. Adipose tissue-derived stem cells are easily available in large numbers and this has made adipose tissue-derived stem cells a potential alternative source of stem cells in the treatment of myocardial infarction. Hypoxia during culture can affect stem cell proliferation, differentiation and paracrine activity.   Hypotheses Human adipose tissue-derived stem cells and bone marrow-derived stem cells are equally affected by hypoxic culture conditions, regarding proliferation, differentiation in an endothelial direction and secretion of growth factors. Hypoxic preconditioned human adipose tissue-derived stem cells and bone marrow-derived stem cells can equally improve left ventricular function after acute myocardial infarction in rats.   Materials and methods The stem cells will be cultured under different oxygen tensions and proliferation will be monitored by DNA quantification using PicoGreen. Paracrine activity will be measured using ELISA kits (VEGF, IGF-1 and HGF) and real time qPCR (VEGF, IGF-1 and Sfrp2). Endothelial differentiation will be evaluated by real time qPCR (VEGF-R2, vWF and CD31), a tube formation assay and intracellular calcium measurements by a Fura-2A based method. The functional effect of human stem cell transplantation after myocardial infarction will be studied by echocardiography, BNP measurements and histology, in a rat model of heart failure following acute myocardial infarction.  Perspectives The study will increase knowledge of hypoxic preconditioning of stem cells and the use of adipose tissue-derived stem cells in the treatment of myocardial infarction. 

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Stem cell therapy following acute myocardial infarction:Comparative studies with adipose tissue-derived stem cells and bone marrow-derived stem cells

https://vbn.aau.dk/ws/files/19254952/abstract

Stem cell therapy following acute myocardial infarction:comparative studies with adipose tissue-derived stem cells and bone marrow-derived stem cells

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