Intermittent low temperature storage is expected to be a new artificial flower-inducing treatment and becoming to be a practical procedure as it does not require expensive equipment or much energy cost. Tray-grown ‘Nyoho’ plants were placed in a refrigerator (15°C, in the dark) for 3 days and then transferred to a further outdoor shelter for 3 days. Plants were transferred at noon and this cycle was repeated twice (3D/3D). Such 3D/3D treatments were begun on August 22, 25, 28, 31, and September 3and the effectiveness on flowering was determined for 2 years. Both in 2012 and 2013, treatments begun before August 28 were less effective compared to the treatments begun on August 28 or later. Continuous 6 days of 15°C storage (6D) was effective to some extent, but 6D from August 25 was apparently less effective compared to those begun later. Both in intermittent and continuous treatments for ‘Nyoho’, it was confirmed that any flower-inducing effect became relatively small and unstable when the treatments were begun before 28 August in Okayama
