Astrocytes are multipotent and serve surprisingly large and diverse variety of functions, providing for the overall brain homeostasis, assisting in neurogenesis, determining the microarchitecture of the grey matter, and defending the brain through evolutionary conserved astrogliosis programs. Astrocytes are specifically involved in various neurodegenerative diseases, including Alzheimer’s and Parkinson’s diseases, and various forms of dementia. Homocysteine is a nonessential sulphur-containing amino acid that had been linked with neurodegenerative diseases and aging

Blahovcova, E.

Halašova, E.

Mahmood, S.

Sopkova, J.

Strnadel, J.

Škovierova, H.

Electronic Sumy State University Institutional Repository

Міністерство освіти та науки України Сумський державний університет Медичний інституту          АКТУАЛЬНІ ПИТАННЯ ТЕРЕТИЧНОЇ ТА ПРАКТИЧНОЇ МЕДИЦИНИ  Topical Issues of Clinical and Theoretical Medicine     Збірник тез доповідей  ІV Міжнародної науково-практичної конференції Студентів та молодих вчених (Суми, 21-22 квітня 2016 року)    ТОМ 1   Суми Сумський державний університет 2016    52 СЕКЦІЯ «ЕКСПЕРИМЕНТАЛЬНА МЕДИЦИНА І» silvered. The scanograms of the deposits’ surfaces were obtained by the means of raster scanning microscopy. Research results. The results showed that the calcifications are built with well-crystallized apatite. Received scanograms clearly show general dependencies in structure of deposits: surface facing walls of blood vessels is smooth and the one that is facing vascular lumen is rough. Therefore smooth, convex surface has a dense nature and less cracks while rough surface has a high amplitude terrain with a clear focus of crystallization. The inner surface of deposits has a crystalline layer of small aggregates. After sonication the layer of small crystals became less sinewy, more pores appeared on rough surface. The presence of pores in the structure of deposits may indicate the participation of organic compounds in the process of formation of pathological biominerals. Conclusions. Raster scanning microscopy made it possible to explore the structure of calcificates. Deposits have a layered structure. Calcificates have two walls: the inner one has no signs of ordered structure while the outer one is smooth and convex. The presence of pores in the structure proves its heterogenic structure mainly caused by organic components involved into the formation of calcificate.   HOMOCYSTENE AND HUMAN ASTROCYTES Škovierová H.1, Mahmood S.1, Blahovcová E.1, Strnádel J.1, Sopková J. 1, Halašová E. 1,2 Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), 1Biomedical Center Martin (BioMed Martin) JFM CU and 2Department of Medical Biology JFM CU, Slovakia Astrocytes are multipotent and serve surprisingly large and diverse variety of functions, providing for the overall brain homeostasis, assisting in neurogenesis, determining the microarchitecture of the grey matter, and defending the brain through evolutionary conserved astrogliosis programs. Astrocytes are specifically involved in various neurodegenerative diseases, including Alzheimer’s and Parkinson’s diseases, and various forms of dementia. Homocysteine is a nonessential sulphur-containing amino acid that had been linked with neurodegenerative diseases and aging. It has been shown, that an increased plasma homocysteine level is an independent risk factor for the development of dementia, Alzheimer’s and Parkinson’s diseases. Homocysteine behaves as an excitatory molecule which markedly enhanced the vulnerability of neuronal cells to exitotoxic, apoptotic, and oxidative injury in vivo and in vitro. However, data about the neurotoxic effect of homocysteine on human astrocytes are lacking. Therefore, we decided to investigate the effect of homocysteine on cultured human astrocytes. We tested cell viability by various tests (MTT and Annexin tests). Homocysteine negatively affected the cell viability in dose-dependent manner, which was evaluated by decreased cell density and lowered ability of cells to metabolize MTT. However, the molecular mechanisms by which HCY induced neurotoxicity are still unknown. Targeting of astroglia may provide a new principle for treatment of neurodegenerative diseases, especially at early stages of AD. This work was supported by the project “Biomedical Center Martin”, ITMS code: 26220220187, the project is co-financed from EU sources.   CHARACTERIZATION OF DENTAL TISSUE DERIVED STEM CELLS Sopková J.1, Blahovcová E.2, Škovierová H.2, Strnádel J.2, Halašová E.2,3 Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), 1Department of Medical Biochemistry, 2Biomedical Center Martin JFM CU and 3Department of Medical Biology, Martin, Slovakia Stem cells (SCs) are undifferentiated cells that are capable to differentiate into more specialized cells with specific functions. Oral tissues, which are easily accessible for dentists are a rich source of stem cells. The isolation of stem cells from these location may still not be convenient, because most of them requires surgical procedures, tooth or pulp extraction. Furthermore, these SCs 

Homocystene and human astrocytes

SocioEconomic Challenges Journal (Sumy State University)

Міністерство освіти та науки України 
Сумський державний університет 
Медичний інституту 
 
 
 
 
 
 
 
 
 
АКТУАЛЬНІ ПИТАННЯ ТЕРЕТИЧНОЇ ТА 
ПРАКТИЧНОЇ МЕДИЦИНИ 
 
Topical Issues of Clinical and Theoretical 
Medicine 
 
 
 
 
Збірник тез доповідей  
ІV Міжнародної науково-практичної конференції 
Студентів та молодих вчених 
(Суми, 21-22 квітня 2016 року) 
 
 
 
ТОМ 1 
 
 
Суми 
Сумський державний університет 
2016 
  
 
52 СЕКЦІЯ «ЕКСПЕРИМЕНТАЛЬНА МЕДИЦИНА І» 
silvered. The scanograms of the deposits’ surfaces were obtained by the means of raster scanning 
microscopy. 
Research results. The results showed that the calcifications are built with well-crystallized 
apatite. Received scanograms clearly show general dependencies in structure of deposits: surface 
facing walls of blood vessels is smooth and the one that is facing vascular lumen is rough. 
Therefore smooth, convex surface has a dense nature and less cracks while rough surface has a high 
amplitude terrain with a clear focus of crystallization. The inner surface of deposits has a crystalline 
layer of small aggregates. After sonication the layer of small crystals became less sinewy, more 
pores appeared on rough surface. The presence of pores in the structure of deposits may indicate the 
participation of organic compounds in the process of formation of pathological biominerals. 
Conclusions. Raster scanning microscopy made it possible to explore the structure of 
calcificates. Deposits have a layered structure. Calcificates have two walls: the inner one has no 
signs of ordered structure while the outer one is smooth and convex. The presence of pores in the 
structure proves its heterogenic structure mainly caused by organic components involved into the 
formation of calcificate. 
 
 
HOMOCYSTENE AND HUMAN ASTROCYTES 
Škovierová H.1, Mahmood S.1, Blahovcová E.1, Strnádel J.1, Sopková J. 1, Halašová E. 1,2 
Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), 
1
Biomedical Center Martin (BioMed Martin) JFM CU and 
2
Department of Medical Biology JFM 
CU, Slovakia 
Astrocytes are multipotent and serve surprisingly large and diverse variety of functions, 
providing for the overall brain homeostasis, assisting in neurogenesis, determining the 
microarchitecture of the grey matter, and defending the brain through evolutionary conserved 
astrogliosis programs. Astrocytes are specifically involved in various neurodegenerative diseases, 
including Alzheimer’s and Parkinson’s diseases, and various forms of dementia. Homocysteine is a 
nonessential sulphur-containing amino acid that had been linked with neurodegenerative diseases 
and aging. It has been shown, that an increased plasma homocysteine level is an independent risk 
factor for the development of dementia, Alzheimer’s and Parkinson’s diseases. Homocysteine 
behaves as an excitatory molecule which markedly enhanced the vulnerability of neuronal cells to 
exitotoxic, apoptotic, and oxidative injury in vivo and in vitro. However, data about the neurotoxic 
effect of homocysteine on human astrocytes are lacking. Therefore, we decided to investigate the 
effect of homocysteine on cultured human astrocytes. We tested cell viability by various tests (MTT 
and Annexin tests). Homocysteine negatively affected the cell viability in dose-dependent manner, 
which was evaluated by decreased cell density and lowered ability of cells to metabolize MTT. 
However, the molecular mechanisms by which HCY induced neurotoxicity are still unknown. 
Targeting of astroglia may provide a new principle for treatment of neurodegenerative diseases, 
especially at early stages of AD. 
This work was supported by the project “Biomedical Center Martin”, ITMS code: 
26220220187, the project is co-financed from EU sources. 
 
 
CHARACTERIZATION OF DENTAL TISSUE DERIVED STEM CELLS 
Sopková J.1, Blahovcová E.2, Škovierová H.2, Strnádel J.2, Halašová E.2,3 
Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin (JFM CU), 
1
Department of Medical Biochemistry, 
2
Biomedical Center Martin JFM CU and 
3
Department of 
Medical Biology, Martin, Slovakia 
Stem cells (SCs) are undifferentiated cells that are capable to differentiate into more 
specialized cells with specific functions. Oral tissues, which are easily accessible for dentists are a 
rich source of stem cells. The isolation of stem cells from these location may still not be convenient, 
because most of them requires surgical procedures, tooth or pulp extraction. Furthermore, these SCs 


English

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Homocystene and human astrocytes

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