Jaeckle, Faculty Advisor, William

Patel, Ketaki K.

Digital Commons @ Illinois Wesleyan University

Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 2000, 11th Annual JWP ConferenceApr 15th, 10:00 AM - 11:00 AMAre there Differences in Nutrient Assimilationamong Cell-Lineages of Sea Urchin Embryos?Ketaki K. PatelIllinois Wesleyan UniversityWilliam Jaeckle, Faculty AdvisorIllinois Wesleyan UniversityFollow this and additional works at: http://digitalcommons.iwu.edu/jwprcThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Ketaki K. Patel and William Jaeckle, Faculty Advisor, "Are there Differences in Nutrient Assimilation among Cell-Lineagesof Sea Urchin Embryos?" (April 15, 2000). John Wesley Powell Student Research Conference. Paper 19.http://digitalcommons.iwu.edu/jwprc/2000/posters/19THE JOHN WESLEY POWELL STUDENT RESEARCH CONFERENCE • APRIL 2000 Poster Presentation 39 ARE THERE DIFFERENCES IN NUTRIENT ASSIMILATION AMONG CELL-LINEAGES OF SEA URCIDN EMBRYOS? Ketaki K. Patel and William laeckle* Department of Biology, lllinois Wesleyan University Embryos of the sea urchins Arbacia punctulata and Lytechinus variegatus were used to test the hypothesis that differences exist among specific cell-lineages in the ability to assimilate nutrients from seawater. Embryos at different developmental stages (from unfertilized eggs to prism stage larvae) were incubated in a seawater solution of the iron-containing protein ferritin (2 mg/mI) for fixed time periods. Following each incubation period, specimens were fixed in neutral buffered formalin. To detect the presence of iron (from ferritin) in cells, experimental specimens and individuals not exposed to ferritin (controls) were incubated in a 3:2 mixture of 1 % HCl and 2% Potassium ferrocyanide. The formation of a blue reaction product revealed those cells containing iron. Results indicate that the ability to assimilate nutrients is detectable between the 8-cell stage and a multicellular pre-blastula stage; and, assimilation is uniform among different cell-lineages. 

Are there Differences in Nutrient Assimilation among Cell-Lineages of Sea Urchin Embryos?

http://digitalcommons.iwu.edu/cgi/viewcontent.cgi?article=1641&context=jwprc

Are there Differences in Nutrient Assimilation among Cell-Lineages of Sea Urchin Embryos?

Abstract

Similar works

Full text

Available Versions

Digital Commons @ Illinois Wesleyan University