Demirjian, Faculty Advisor, David

Hoffmann, Faculty Advisor, Stephen

Tisoncik, \u2707, Melissa

Digital Commons @ Illinois Wesleyan University

Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 2007, 18th Annual JWP ConferenceApr 14th, 2:35 PM - 3:35 PMSynthesis of Rare Sugar Molecules: UtilizingFermentation Biotechnology for the Production ofL-RiboseMelissa Tisoncik, '07Illinois Wesleyan UniversityStephen Hoffmann, Faculty AdvisorIllinois Wesleyan UniversityDavid Demirjian, Faculty AdvisorZuchem, Inc.Follow this and additional works at: http://digitalcommons.iwu.edu/jwprcThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Melissa Tisoncik, '07; Stephen Hoffmann, Faculty Advisor; and David Demirjian, Faculty Advisor, "Synthesis of RareSugar Molecules: Utilizing Fermentation Biotechnology for the Production of L-Ribose" (April 14, 2007). John WesleyPowell Student Research Conference. Paper 19.http://digitalcommons.iwu.edu/jwprc/2007/posters2/19THE JOHN WESLEY POWELL STUDENT RESEARCH CONFERENCE - APRIL 2007 Poster Presentation P78 SYNTHESIS OF RARE SUGAR MOLECULES: UTILIZING FERMENTATION BIOTECHNOLOGY FOR THE PRODUCTION OF L-RIBOSE Melissa Tisoncik and Stephen Hoffmann* and David Demirjian* Chemistry Department, Illinois Wesleyan University and Zuchem, Inc. Carbohydrate-based molecular research for biochemical and pharmaceutical companies is becoming increasingly important due to the i nvolvement of carbohydrates in numerous biological functions; including cellular recognition, signaling, and even the development of disease states. The need to access consistent, pure and inexpensive carbohydrate starting materials is also an important factor to consider. This research is focused on the synthesis of one such rare glycochemical L-ribose due to its use in nucleoside-based pharmaceuticals. Zuchem, a biochemical company,  has proposed a new synthetic route for the production of L-ribose which utilizes the NAD-dependent mannitol dehydrogenase (MDH) from Apium graveolens (garden celery) by specifically converting ribitol to L-ribose through the means of enzymatic oxidation using proprietary fermentation biotechnology. To increase optimization and purity of the L­ribose product through this synthetic route, Zuchem research parameters involve varying MDH zinc dependency, fermentation salt concentrations, and utilizing numerous inexpensive starting materials. In conclusion, this process produced about 90% pure L-ribose fractions for marketing. 

Synthesis of Rare Sugar Molecules: Utilizing Fermentation Biotechnology for the Production of L-Ribose

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Synthesis of Rare Sugar Molecules: Utilizing Fermentation Biotechnology for the Production of L-Ribose

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