Maroun, Faculty Advisor, Leonard

Zook, Nicole

Digital Commons @ Illinois Wesleyan University

Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 1992, 3rd Annual JWP ConferenceApr 25th, 10:30 AM - 4:30 PMIn Situ Hybridization Screening of a LambdaLibrary for Chromosome Specific DNA: TheIsolation of a Rabbit Chromosome Number 9ProbeNicole ZookIllinois Wesleyan UniversityLeonard Maroun, Faculty AdvisorSouthern Illinois University CarbondaleFollow this and additional works at: http://digitalcommons.iwu.edu/jwprcThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Nicole Zook and Leonard Maroun, Faculty Advisor, "In Situ Hybridization Screening of a Lambda Library for ChromosomeSpecific DNA: The Isolation of a Rabbit Chromosome Number 9 Probe" (April 25, 1992). John Wesley Powell StudentResearch Conference. Paper 1.http://digitalcommons.iwu.edu/jwprc/1992/posters/11 IN SITU HYBRIDIZATION SCREENING OF A LAMBDA LIBRARY FOR CHROMOSOME SPECIFIC DNA: THE ISOLATION OF A RABBIT CHROMOSOME NUMBER 9 PROBE 2 Nicole Zook, Dept. of Biology, IWU/Dept. of Medical Microbiology & Immunology, Southern illinois University School of Medicine, Leonard Maroun* 3 Downs syndrome, a disease which effects 1 of every 783 newborns, is the result of anueploidy of human chromosome 2 1. The closely related rabbit chromosome 20 can be used as a model for the study of the cause of the misdistribution of this chromosome during meiosis. The procedure, called karyotyping, requires long hours behind the microscope; however, it can be replaced with the more accurate, time-efficeint restriction fragment length polymorphism (RFLP) assay. The purpose of this study was to test the feasability of finding a chromosome specific probe by searching a lambdda library using IN SITU hybridization. IN SITU hybridization involves annealing a radioactive DNA probe directly to metaphase chromosomes fixed to a microscope slide. A photographic emulsion is spread over the slides. Photographic grains appear over the chromosome to which the DNA has annealed thus identifying the chromosome from which the probe DNA originated (FIG. 1) .  Probe DNA was phenol extracted from lambda phage virus clones that carry rabbit DNA inserts. Inserts were purified by gel electrophoresis (FIG. 2) .  Using this procedure, a chromosome 9 probe was discovered in the 86 phage DNA clones isolated (FIG. 3 ) .  This suggests that a larger search would be capable of finding the chromosome 20 probe needed to develop a rabbit model for Downs syndrome. 

In Situ Hybridization Screening of a Lambda Library for Chromosome Specific DNA: The Isolation of a Rabbit Chromosome Number 9 Probe

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In Situ Hybridization Screening of a Lambda Library for Chromosome Specific DNA: The Isolation of a Rabbit Chromosome Number 9 Probe

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