Eze-Echesi, Golibe

Walter, Faculty Advisor, Brian

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Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 2010, 21st Annual JWP ConferenceApr 10th, 2:35 PM - 3:35 PMUse of Slide In-Situ Hybridization to DeterminePAX9 Gene Expression in MoenkhausiaSanctaefilomenaeGolibe Eze-EchesiIllinois Wesleyan UniversityBrian Walter, Faculty AdvisorIllinois Wesleyan UniversityFollow this and additional works at: http://digitalcommons.iwu.edu/jwprcPart of the Biology CommonsThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Eze-Echesi, Golibe and Walter, Faculty Advisor, Brian, "Use of Slide In-Situ Hybridization to Determine PAX9 GeneExpression in Moenkhausia Sanctaefilomenae" (2010). John Wesley Powell Student Research Conference. 8.http://digitalcommons.iwu.edu/jwprc/2010/posters2/8THE JOHN WESLEY POWELL STUDENT RESEARCH CONFERENCE - APRIL 2010 Poster Presentation P16 USE OF SLIDE IN-SITU HYBRIDIZATION TO DETERMINE PAX9 GENE EXPRESSION IN MOENKHAUSIA SANCTAEFILOMENAE GoUbe Eze-Echesi and BrianWalter* Biology Department, Illinois Wesleyan University Pax9 is a paired box transcription factor with essential roles in embryonic development of the skeleton, teeth, thymus and parathyroid regions of the embryo. Our lab has previously cloned Pax9 from the red eye tetra, Moenkhausia sanctaefilomenae and expression analysis during early embryonic development demonstrated that Pax9 is expressed in the pharyngeal region of the craniofacial skeleton, vertebral somites and tail region. This project examines expression of Pax9 in larval fish using a technique known as slide in-situ hybridization. This technique allows for detection of Pax9 transcripts in older specimens, an outcome typically unattainable via whole mount in-situ techniques. Collagen type II alpha} (CoI2a}) was also examined as a control for this technique. Results showed that Col2a} is expressed in the basioccipital cartilage and the chondocranium cartilage while pax9 is expressed in the tissues of the pancreas and liver of the embryo. The success of this slide in-situ technique allows for further examination of later development in fish while possibly using other genes as well. 

Use of Slide In-Situ Hybridization to Determine \u3cem\u3ePAX9\u3c/em\u3e Gene Expression in \u3cem\u3eMoenkhausia Sanctaefilomenae\u3c/em\u3e

https://digitalcommons.iwu.edu/cgi/viewcontent.cgi?article=2537&amp;context=jwprc

Use of Slide In-Situ Hybridization to Determine \u3cem\u3ePAX9\u3c/em\u3e Gene Expression in \u3cem\u3eMoenkhausia Sanctaefilomenae\u3c/em\u3e

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