Newborns with very low and extremely low body weight at birth (VLBW and ELBW) are
the most complicated category of patients in a Department of resuscitation and intensive therapy of newborns (ICU). Objective: to determine of colonization by opportunistic microorganisms and fungi, as well as their sensitivity in newborns in ICU

Asal, Issa

Muhina, Ibrahim Ali

SocioEconomic Challenges Journal (Sumy State University)

 3  
Міністерство освіти та науки України 
Сумський державний університет 
Медичний інституту 
 
 
 
 
 
 
 
 
 
АКТУАЛЬНІ ПИТАННЯ ТЕРЕТИЧНОЇ ТА 
ПРАКТИЧНОЇ МЕДИЦИНИ 
 
Topical Issues of Clinical and Theoretical 
Medicine 
 
 
 
 
Збірник тез доповідей  
ІV Міжнародної науково-практичної конференції 
Студентів та молодих вчених 
(Суми, 21-22 квітня 2016 року) 
 
 
 
ТОМ 2 
 
 
Суми 
Сумський державний університет 
2016 
  
 216 СЕКЦІЯ «ПЕДІАТРІЯ З ДИТЯЧИМИ ІНФЕКЦІЯМИ» 
effectiveness of a hypoallergenic diet therapy (HD) products based on the full or partial hydrolysis 
of serum protein in cow's milk (CMP) in infants with symptoms of atopic dermatitis (AD).  
Integrated dynamic, within 2 months of observation conducted in 31 infant child that was 
bottle-fed, had clinical signs of atopic dermatitis. With the use of tables of random numbers were 
distributed on two clinical comparison group. The first (I) clinical group entered 21patient, which 
was designed soft hypoallergenic diet therapy blends "HiPP HA1 combiotik" and "HiPP HA2 
combiotik" (according to age and severity of manifestations of AD). To the second group (group) 
included 10 infants who were administered hypoallergenic mix of other manufacturers (optional 
mother). 
Duration of observation was 2 months. On the main clinical characteristics were comparable 
groups. Following the correction of malnutrition among children in group I mean score on the EASI 
scale decreased by 2,2 times (from 12,6±2,6 to 5,7±0,9 points (P<0,05)), where as in infants group 
II - is only 1,6 times (from 3,8±1,1 to 2,3±0,6 score (P<0,05)). In the beginning of the observation 
points indicated amount was recorded in 85,7% of children in I group and after - only 57,1 % of 
patients (Pφ<0,05). In II group, the proportion of such patients decreased from 20,0 % to 10,0 % of 
children (Pφ>0,05). As a result of consistent HD children showed decrease in symptom severity AD 
57,1±10,8% of cases, where as patients II group - only 10,0±9,4% of observations (P<0,05). The 
relative risk reduction EASI score on a scale of 4,4 points or higher in patients of group versus 
control was 47,1% absolute risk reduction – 52,3% with a minimum number of patients to be treat 
to obtain one positive result - 2. 
Thus the product "HiPP HA1 combiotic» recommended as a starting formula feeding for 
children allergic to cow's milk proteins are easy and moderate to anyone under 1 year of age, 
starting of the first days of life, and for the necessary term to stabilize the clinical effect and 
subsiding manifestations of atopic dermatitis. 
 
 
MICROBIOLOGICAL RESEARCH IN NEONATAL ICU 
(SUMY REGION CHILDREN'S CLINICAL HOSPITAL) 
Muhina Ibrahim Ali, - 6
th
 y. student, 029 gr., Issa Asal, - 6
th
 y. student, 019 gr. 
Scientific supervisor – associate professor E.K. Redko 
Sumy State University 
Department of Pediatrics with Medical Genetics 
Newborns with very low and extremely low body weight at birth (VLBW and ELBW) are 
the most complicated category of patients in a Department of resuscitation and intensive therapy of 
newborns (ICU).  
Objective: to determine of colonization by opportunistic microorganisms and fungi, as well 
as their sensitivity in newborns in ICU.  
Results. In 60%, samples of the cultures material were positive (+). Coagulase-Negative St. 
(CoNS) (48/100 +); with mucous membranes of neonates most often stood. 58% of CoNS isolates 
methicillin-resistant were, respectively. Representatives of the Enterobacteriaceae were inoculated 
with a frequency of 16/100 +: a total of 268 isolates, 15% was resistant to Cephalosporin I–IV 
generation, and 0.7 % r to Carbapenem. The trend of decrease in the isolation rate of non-
fermentative gram-negative rods up to 2.5/100 + noted. They were represented only by two species 
of Pseudomonas aeruginosa and Acinetobacter spp. and had a wide spectrum of antimicrobial 
sensitivity. 618 samples of blood cultures from 453 children were studied, of whom 6% +.  Fungi 
represented by 3 genera: Candida, Malassezia and Saccoharomyces. The most frequently inoculated 
Candida – 1.4% and Malassezia –1%. 70% from fungi - Candida albicans sensitive to fluconazole. 
Conclusion. 1. Microbiological monitoring enables to monitor the stages and features of the CPM 
colonization of the newborns in the hospital.   Individual approaches to each patient undergoing 
treatment in ICU, conducting flexible antibioticotherapia reduce the use of antimicrobials reserve 
and to reduce the frequency of colonization in children fungal flora.  
2. On the background of reducing the frequency of use of antibiotics of a reserve in 
dynamics is clearly a trend towards reducing the frequency of colonization of newborns no 
 217 СЕКЦІЯ «ПЕДІАТРІЯ З ДИТЯЧИМИ ІНФЕКЦІЯМИ» 
fermentative microorganisms. The results are similar to the trends observed in ORAN of most of 
Europe.  
 
 
STRUCTURAL AND FUNCTIONAL CONDITION OF LIVER OF CHILDREN WITH 
ESCHERICHIOSIS 
INFECTED BY EPSTEIN-BARR VIRUS 
Olkhovskyy E.S. 
Scientific head - prof. Kuznetsov S.V. 
Kharkiv National Medical University, Department of Children Infectious Diseases. 
From year to year the frequency of combined infections increases in pediatrician practice. 
One of the most common infections are Herpes viral and bacterial intestinal infection, including - 
Escherichiosis. The course of disease is basically determined by the condition of internal organs, 
especially - the liver. 
Purpose of investigation to study structural and functional condition of the liver in children 
with Escherichiosis, infected by Epstein-Barr virus (EBV). 
The liver ultrasound investigation and liver function test in 64 children 1 mo - 3 years with 
moderate forms of Escherichiosis were done. Among all patients 25 children with persistent EBV 
infection have been isolated (co-infection).The parenchymal reaction of liver with increased 
echogenicity to 10-12 gradation was found in 20 (80%) patients, the signs of hepatosplenitis in 4 
patients (16%), liver gate lymphadenitis in 16 patients (64%), mesadenitis in 12 patients (48%) with 
co-infection. An examination of patients with Escherichiosis (mono-infection) revealed 
parenchymal reactions of liver only in 10 patients (25,64%) with a slight increase of tissue 
echogenicity. There was only one case of hepatitis among patients with mono-infection, and no 
inflammation of the lymph nodes. Comparison of liver function test revealed increase of ALT in 13 
children (52%) with co-infection. The level of ALT exceeded the physiological parameters not 
more than one and a half times. All patients with Escherichiosis without EBV infection have normal 
rates of the liver test. 
Thus Escherichiosis in young children with persistent EBV infection is accompanied by 
changes of liver structural and functional condition that must be considered at all stages of 
treatment. 
 
 
EFFECTS OF EXPERIMENTAL HYPOXIA ON IRON BALANCE IN THE BRAIN 
TISSUE OF RATS 
Samee Al Musa, Sadid Laith  - 5
th
 year students 
Scientific supervisor - associate professor Petrashenko V.O. 
Sumy State University, Department of Pediatrics with Medical Genetics 
Hypoxic-ischemic lesion is one of important problems of neonatology, which is determined 
by their place in the structure of morbidity, perinatal mortality and a value in the disorders 
formation. Microelements provide course of important biological reactions and are catalysts of 
many of them. 
Research purpose: to research iron cerebral tissue supply in case of experimental hypoxia 
with different degrees of complexity.  
Microelement supply for iron was studied as well as the lead level of cerebral tissue in 
experimental hypoxia conditions. Microelement supply was investigated on 44 laboratory rodents 
on their first and seventh days. 
The newborn rats had a high level of iron in their cerebral tissues like 571,5±1,5 mkg/g. 
Microelement level was rapidly reduced after a week of their born. It might be caused by high usage 
of tissue iron during oxidative reactions and energy generation processes. In this case the iron level 
was just 58,33±1,09 mkg/g.  
The correlation analysis of contained iron level in animal organs on their first alive day 
pointed out that the level of element correlation in brain with iron level of other organs is quiet 
weak (r= -0,28 for the liver) or totally absent (r= 0,12 for the kidneys and r= -0,07 for the heart).  


Microbiological research in neonatal ICU (Sumy region children's clinical hospital)

Electronic Sumy State University Institutional Repository

 3  Міністерство освіти та науки України Сумський державний університет Медичний інституту          АКТУАЛЬНІ ПИТАННЯ ТЕРЕТИЧНОЇ ТА ПРАКТИЧНОЇ МЕДИЦИНИ  Topical Issues of Clinical and Theoretical Medicine     Збірник тез доповідей  ІV Міжнародної науково-практичної конференції Студентів та молодих вчених (Суми, 21-22 квітня 2016 року)    ТОМ 2   Суми Сумський державний університет 2016    216 СЕКЦІЯ «ПЕДІАТРІЯ З ДИТЯЧИМИ ІНФЕКЦІЯМИ» effectiveness of a hypoallergenic diet therapy (HD) products based on the full or partial hydrolysis of serum protein in cow's milk (CMP) in infants with symptoms of atopic dermatitis (AD).  Integrated dynamic, within 2 months of observation conducted in 31 infant child that was bottle-fed, had clinical signs of atopic dermatitis. With the use of tables of random numbers were distributed on two clinical comparison group. The first (I) clinical group entered 21patient, which was designed soft hypoallergenic diet therapy blends "HiPP HA1 combiotik" and "HiPP HA2 combiotik" (according to age and severity of manifestations of AD). To the second group (group) included 10 infants who were administered hypoallergenic mix of other manufacturers (optional mother). Duration of observation was 2 months. On the main clinical characteristics were comparable groups. Following the correction of malnutrition among children in group I mean score on the EASI scale decreased by 2,2 times (from 12,6±2,6 to 5,7±0,9 points (P<0,05)), where as in infants group II - is only 1,6 times (from 3,8±1,1 to 2,3±0,6 score (P<0,05)). In the beginning of the observation points indicated amount was recorded in 85,7% of children in I group and after - only 57,1 % of patients (Pφ<0,05). In II group, the proportion of such patients decreased from 20,0 % to 10,0 % of children (Pφ>0,05). As a result of consistent HD children showed decrease in symptom severity AD 57,1±10,8% of cases, where as patients II group - only 10,0±9,4% of observations (P<0,05). The relative risk reduction EASI score on a scale of 4,4 points or higher in patients of group versus control was 47,1% absolute risk reduction – 52,3% with a minimum number of patients to be treat to obtain one positive result - 2. Thus the product "HiPP HA1 combiotic» recommended as a starting formula feeding for children allergic to cow's milk proteins are easy and moderate to anyone under 1 year of age, starting of the first days of life, and for the necessary term to stabilize the clinical effect and subsiding manifestations of atopic dermatitis.   MICROBIOLOGICAL RESEARCH IN NEONATAL ICU (SUMY REGION CHILDREN'S CLINICAL HOSPITAL) Muhina Ibrahim Ali, - 6th y. student, 029 gr., Issa Asal, - 6th y. student, 019 gr. Scientific supervisor – associate professor E.K. Redko Sumy State University Department of Pediatrics with Medical Genetics Newborns with very low and extremely low body weight at birth (VLBW and ELBW) are the most complicated category of patients in a Department of resuscitation and intensive therapy of newborns (ICU).  Objective: to determine of colonization by opportunistic microorganisms and fungi, as well as their sensitivity in newborns in ICU.  Results. In 60%, samples of the cultures material were positive (+). Coagulase-Negative St. (CoNS) (48/100 +); with mucous membranes of neonates most often stood. 58% of CoNS isolates methicillin-resistant were, respectively. Representatives of the Enterobacteriaceae were inoculated with a frequency of 16/100 +: a total of 268 isolates, 15% was resistant to Cephalosporin I–IV generation, and 0.7 % r to Carbapenem. The trend of decrease in the isolation rate of non-fermentative gram-negative rods up to 2.5/100 + noted. They were represented only by two species of Pseudomonas aeruginosa and Acinetobacter spp. and had a wide spectrum of antimicrobial sensitivity. 618 samples of blood cultures from 453 children were studied, of whom 6% +.  Fungi represented by 3 genera: Candida, Malassezia and Saccoharomyces. The most frequently inoculated Candida – 1.4% and Malassezia –1%. 70% from fungi - Candida albicans sensitive to fluconazole. Conclusion. 1. Microbiological monitoring enables to monitor the stages and features of the CPM colonization of the newborns in the hospital.   Individual approaches to each patient undergoing treatment in ICU, conducting flexible antibioticotherapia reduce the use of antimicrobials reserve and to reduce the frequency of colonization in children fungal flora.  2. On the background of reducing the frequency of use of antibiotics of a reserve in dynamics is clearly a trend towards reducing the frequency of colonization of newborns no  217 СЕКЦІЯ «ПЕДІАТРІЯ З ДИТЯЧИМИ ІНФЕКЦІЯМИ» fermentative microorganisms. The results are similar to the trends observed in ORAN of most of Europe.    STRUCTURAL AND FUNCTIONAL CONDITION OF LIVER OF CHILDREN WITH ESCHERICHIOSIS INFECTED BY EPSTEIN-BARR VIRUS Olkhovskyy E.S. Scientific head - prof. Kuznetsov S.V. Kharkiv National Medical University, Department of Children Infectious Diseases. From year to year the frequency of combined infections increases in pediatrician practice. One of the most common infections are Herpes viral and bacterial intestinal infection, including - Escherichiosis. The course of disease is basically determined by the condition of internal organs, especially - the liver. Purpose of investigation to study structural and functional condition of the liver in children with Escherichiosis, infected by Epstein-Barr virus (EBV). The liver ultrasound investigation and liver function test in 64 children 1 mo - 3 years with moderate forms of Escherichiosis were done. Among all patients 25 children with persistent EBV infection have been isolated (co-infection).The parenchymal reaction of liver with increased echogenicity to 10-12 gradation was found in 20 (80%) patients, the signs of hepatosplenitis in 4 patients (16%), liver gate lymphadenitis in 16 patients (64%), mesadenitis in 12 patients (48%) with co-infection. An examination of patients with Escherichiosis (mono-infection) revealed parenchymal reactions of liver only in 10 patients (25,64%) with a slight increase of tissue echogenicity. There was only one case of hepatitis among patients with mono-infection, and no inflammation of the lymph nodes. Comparison of liver function test revealed increase of ALT in 13 children (52%) with co-infection. The level of ALT exceeded the physiological parameters not more than one and a half times. All patients with Escherichiosis without EBV infection have normal rates of the liver test. Thus Escherichiosis in young children with persistent EBV infection is accompanied by changes of liver structural and functional condition that must be considered at all stages of treatment.   EFFECTS OF EXPERIMENTAL HYPOXIA ON IRON BALANCE IN THE BRAIN TISSUE OF RATS Samee Al Musa, Sadid Laith  - 5th year students Scientific supervisor - associate professor Petrashenko V.O. Sumy State University, Department of Pediatrics with Medical Genetics Hypoxic-ischemic lesion is one of important problems of neonatology, which is determined by their place in the structure of morbidity, perinatal mortality and a value in the disorders formation. Microelements provide course of important biological reactions and are catalysts of many of them. Research purpose: to research iron cerebral tissue supply in case of experimental hypoxia with different degrees of complexity.  Microelement supply for iron was studied as well as the lead level of cerebral tissue in experimental hypoxia conditions. Microelement supply was investigated on 44 laboratory rodents on their first and seventh days. The newborn rats had a high level of iron in their cerebral tissues like 571,5±1,5 mkg/g. Microelement level was rapidly reduced after a week of their born. It might be caused by high usage of tissue iron during oxidative reactions and energy generation processes. In this case the iron level was just 58,33±1,09 mkg/g.  The correlation analysis of contained iron level in animal organs on their first alive day pointed out that the level of element correlation in brain with iron level of other organs is quiet weak (r= -0,28 for the liver) or totally absent (r= 0,12 for the kidneys and r= -0,07 for the heart).  

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Microbiological research in neonatal ICU (Sumy region children's clinical hospital)

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