The magnetic nanocomplex (MNC) comprised of Fe3O4 nanoparticles (NP) and doxorubicin (DOXO) with a saturation magnetic magnetic moment m3 = 10.5 emu/g during magnetic nanotherapy initiated greater antitumor effect than MNC with m3 = 8.55 emu/g. After treatment of Walker 256 tumor the concentration of iron ions in the blood serum of animals with tumor increased and did not depend on the magnetic properties of MNC

Melnik, V.O.

Orel, V.E.

Romanov, A.V.

Rykhalskyi, O.Yu.

Shevchenko, A.D.

SocioEconomic Challenges Journal (Sumy State University)

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE  
NANOMATERIALS: APPLICATIONS AND PROPERTIES 
Vol. 4 No 2, 02NABM01(3pp) (2015) 
 
 
2304-1862/2015/4(2)02NABM01(3) 02NABM01-1  
Magnetic Nanotherapy by Magnetosensitive Nanocomplexes with Different Magnetic  
Properties of the Walker 256 Carcinosarcoma 
 
V.E. Orel1,*, A.D. Shevchenko2, V.O. Melnik1, O.Yu. Rykhalskyi1, A.V. Romanov1 
 
1 National Cancer Institute, 33/43 Lomonosov Str., 03022, Kyiv, Ukraine 
2 G.V. Kurdyumov Institute for Metal Physics, N.A.S. of Ukraine, 36 Academican Vernadsky Boulevard, UA- 03680 
Kyiv-142, Ukraine 
 
(Received 03 April 2015; revised manuscript received 23 July 2015; published online 25 August 2015) 
 
The magnetic nanocomplex (MNC) comprised of Fe3O4 nanoparticles (NP) and doxorubicin (DOXO) with a 
saturation magnetic moment ms  10.5 emu/g during magnetic nanotherapy initiated greater antitumor  
effect than MNC with ms  8.55 emu/g. After treatment of Walker 256 tumor the concentration of iron ions 
in the blood serum of animals with tumor increased and did not depend on the magnetic properties of 
MNC.  
 
Keywords: Nanoparticles, Carcinosarcoma Walker 256, Iron Ions, Blood Serum, Magnetic Nanotherapy. 
 
 PACS numbers: 75.75. + a, 87.85. – d 
 
 
                                                          
*v-orel@voliacable.com 
1. INTRODUCTION 
 
Magnetic nanoparticles (NP) hyperthermia appears 
well-suited as an effective tumor therapy: (1) the con-
centration of NP in the tumor is both sufficiently high 
and significantly higher than in surrounding, normal 
tissue, (2) the use of NP, which can combine both ther-
apeutic and diagnostic capabilities in one dose, has the 
potential to lead toward personalized oncology and bet-
ter outcomes for patients, (3) the particles possess a 
high enough specific absorption rate. However, there 
are a number of limitations of magnetic NP hyper-
thermia such as: (1) therapy by NP in the temperature 
range 43–70 °C can be accompanied by the formation of 
drug resistance due to the induction of heat shock pro-
teins, (2) the temperature above 45 C may shut down 
tumor tissue perfusion, and (3) targeted therapy with 
magnetic NP is often not suitable for disseminated and 
abdominal tumors [1]. 
To overcome the above problems, we have developed 
a new technology of magnetic nanotherapeutics based 
on multiple modes of actions, such as mild hyperther-
mia less 40 C [2]. 
In this paper we studied an influence of magneto-
sensitive nanocomplexes (MNC) with different magnet-
ic properties on the iron distribution in serum of ani-
mals with Walker 256 carcinosarcoma under magnetic 
nanotherapy. 
 
2. MATERIAL AND METHODS 
 
As a parts or drug components the NP of iron oxide 
Fe3O4 in diameter < 50 nm (Sigma-Aldrich) and do-
xorubicin (DOXO) (Pfizer, Italy) have been used. There 
were two types of synthesized samples MNC with dif-
ferent magnetic properties when exposed to 5 mT con-
stant magnetic field (СMF) (MNС1) and 8 mT СMF 
(MNС2). Also, the effects of 5 mT and 8 mT CMF on the 
samples of iron oxide (labeled accordingly Fe3O4-1, 
Fe3O4-2) and DOXO (labeled accordingly DOXO1 and 
DOXO2) were examined during magneto-mechano-
chemical activation. 
The study of antitumor activity of DOXO, MNC, 
CMF and electromagnetic irradiation (EI) was conduct-
ed on 60 rats females weighing (175 ± 14) g from vivar-
ium National Cancer Institute. Transplantation of tu-
mor cells Walker 256 carcinosarcoma was performed by 
administration to the rat right thigh 20% cell suspen-
sion in a volume of 0.4 ml in the medium 199. DOXO 
were injected into animals at a dose of 1.5 mg/kg, MNC: 
DOXO at a dose of 1.5 mg/kg, Fe3O4 at a dose of 3 
mg/kg. Drug administration was carried out in the tail 
vein of animals in a volume of 0.3 ml 0.9% NaCl solu-
tion. Treatment started 3 days after tumor transplan-
tation and was performed five times every other day. 
Local EI of tumor animals was performed by experi-
mental prototype device “Magnetotherm” (Radmir, 
Ukraine) with magnetic-dipole applicator that included 
needle localizer and neodymium permanent magnet 
with a maximum CMF magnetic induction of 0.4 T at a 
distance of 8 mm from the end of the dipoles and EMF 
frequency of 40 MHz with output power of 75 W. Tem-
perature control was carried by fiber-optic thermometer 
TM-4 (Radmir, Ukraine). Intratumoral temperature did 
not exceed 38 C. 
The magnetic properties were studied by magne-
tometery using a “Vibrating Magnetometer 7404 VSM” 
(Lake Shore Cryotronics, Inc., USA) with magnetic 
fields up to 13 kOe. The magnetometer’s sensitivity is 
10-7 emu, and that allowed measurements of magnetic 
moment of samples weighing milligrams to be per-
formed. The mass was determined by an electronic mi-
crobalance AB135-S/FACT with auto-identification 
(Mettler Toledo, Switzerland) which has a sensitivity 
of 10-5 g. 
Colorimetric analysis with the Ferrozine (Ortho-
Clinical Diagnostics, UK) has been used for the meas-
urement of serum iron concentration by Vitros 250 
(USA) chemistry analyzer. 
 V.E. OREL, A.D. SHEVCHENKO ET AL. PROC. NAP 4, 02NABM01 (2015) 
 
 
02NABM01-2 
3. RESULTS AND DISCUSSION 
 
The effect of MNC with different magnetic proper-
ties on the nonlinear kinetics of Walker 256 carcinosar- 
coma growth from the 3rd to the 15th day after tumor 
transplantation is shown at Table 1. According to the 
obtained data, a statistically significant difference was 
observed for anticancer effects of MNC1 as compared to 
MNC2 both without the influence CMF and EMF, and 
after combined action of CMF and EMF. The MNC an-
titumor effect was evaluated on the base of the inhibi-
tion coefficient of tumor growth. The MNC2 antitumor 
effect was 16.5 and 19% higher in the first and second 
case, respectively, as compared to MNC1. 
 
Table 1 – An influence of MNC with different magnetic prop-
erties on the growth kinetics of Walker 256 carcinosarcoma 
after irradiation by CMF and EMF 
 
N Animal Group Parameter 
Growth 
Factor , 
day-1 
Braking 
Ratio , 
Relative 
Units 
1 Control (without 
treatment) 
0.43±0.01 1.00 
2 Conventional 
DOXO 
0.32±0.01 1.35 
3 MNC 1 0.40±0.01 1.09 
4 MNC 2 0.33±0.02 1.31 
5 MNC 1 + CMF + EI 0.34±0.01 1.27 
6 MNC 2 + CMF + EI 0.28±0.02§ 1.56 
, , , § Statistically significant difference from 1, 2, 3, and 5 
animal group, respectively, р < 0.05 
 
When compared antitumor effect of MNC with con-
ventional DOXO it should be noted that only in the 6th 
group of animals after joint action of MNС2 with СMF 
and EMF the braking ratio of tumor growth was in-
creased by 15.5%. In further observation of changing in 
tumor size till 22 days after transplantation the trend 
for the breaking ratio remaining unchanged in spite of 
the fact that some animals with large tumor size in 1–4 
groups were died. 
An analysis of hysteresis loops (Fig. 1, Table 2) indi-
cates that the samples of MNC, Fe3O4, DOXO1 and 
DOXO2 were soft ferromagnets. Conventional DOXO 
has diamagnetic properties. Greater saturation mag-
netic moment and the magnetic hysteresis loop area 
had MNC and DOXO samples synthesised or activated 
in nanoreactor with CMF induction of 8 mT. The coer-
cive force in this case decreased. For Fe3O4-2 unlike 
MNC2 an increase in coercivity and reduction of mag-
netic hysteresis loop were reported as compared with 
Fe3O4-1. 
Iron ions are acceptor of electromagnetic field. 
Magnetic nanotherapy can influence the reaction yields 
of iron ions between the tumor and the blood. To un-
derstand the distribution of the iron in the tumor and 
blood serum after magnetic nanotherapy we have ana-
lyzed the concentration of iron and iron-containing pro-
teins in the serum blood of animals. In Table 3 an in-
fluence of MNC with different magnetic properties on 
iron concentration in blood serum of animals with 
Walker 256 carcinosarcoma after irradiation by CMF 
and EMF on the 15th day after tumor transplantation 
is shown. The content of iron ions in the blood serum 
increased in 2.2 times on the average in all studied 
groups, regardless of received treatment, as compared 
to the control group and animals without tumors. 
A comparison obtained experimental results and 
published in the paper [3] confirms the previously 
known tendency to increase of the iron content in blood 
serum under the influence of chemotherapy of malig-
nant tumors. According to the above cited authors this 
is due to the effect of hemolysis, assuming that 60% of 
iron in the body is found in hemoglobin. 
 
 
 
 a 
 
 
 
 b 
 
 
 
 c 
 
Fig. 1 – Hysteresis loops. MNC (а), Fe3O4 (b) and DOXO (c) at 
38 С. Н – magnetic field; m – magnetic moment. В – CMF 
induction during magneto-mechano-chemical synthesis in 
nanoreactor: 1 – MNC1 (B = 5 mT), 2 – MNC2 (B = 8 mT) 
 
 
 ANTITUMOR EFFECT AND THE DISTRIBUTION … PROC. NAP 4, 02NABM01 (2015) 
 
 
02NABM01-3 
Table 2 – Magnetic properties of the samples at 38 С 
 
Examined object MNC1 MNC2 Fe3O4-1 Fe3O4-2 DOXO1* DOXO2 
CMF induction during magneto-
mechano-chemical activation or synthe-
sis in nanoreactor, mT 
5 8 5 8 5 8 
M
a
g
n
e
ti
c 
h
y
s
te
re
s
is
 
lo
o
p
s 
Saturation magnetic moment 
ms, emu/g 
8.55 10.5 31.8 41.6 0.05 0.37 
Coercive field Hc, Oe 13.1 4.4 15.2 18.62 30.1 24.8 
Area of hysteresis loop, erg/g 136.3 1135.4 5613.3 1657 17 36.7 
*Conventional DOXO is diamagnetic, magnetic moment m = – 1.18 emu/g at 3000 Oe 
 
 
Table 3 – An influence of MNC with different magnetic prop-
erties on the concentration of iron in blood serum of animals 
with Walker 256 carcinosarcoma after irradiation by CMF and 
EMF 
 
N Animal group Iron, μmol/l 
1 Control (without treatment) 15.4±1.2 
2 Conventional DOXO 38.4±2.6 
3 MNC 1 31.0±3.9 
4 MNC 2 38.2±3.4 
5 MNC 1 + CMF + EI 34.1±3.1 
6 MNC 2 + CMF + EI 35.3±4.6 
7 Animals without tumor 16.7±0.77§₤ 
, , , , §, ₤ Statistically significant difference from 1, 2, 3, 4, 5, 
and 6 animal group, respectively, р < 0.05 
 
Thus, it is possible to state that the use of magnetic 
nanotherapy technology opens up the opportunities for 
further implementation in clinical practice the effect of 
the controlled local toxicity based on the selection of 
optimal magnetic properties of MNC. 
 
4. CONCLUSION 
 
Magnetic nanotherapy by the MNC comprised of 
Fe3O4 NP and DOXO with saturation magnetic mo-
ment ms=10.5 emu/g and hysteresis loop area of 1135.4 
erg/g has initiated greater growth inhibition factor of 
Walker-256 carcinosarcoma on 23% as compared to the 
MNC with lesser saturation magnetic moment ms=8.55 
emu/g and hysteresis loop area of 136.3 erg/g. The con-
tent of free iron complexes was minimal in Walker-256 
carcinosarcoma after magnetic nanotherapy by MNC 
with a large hysteresis loop area. The concentration of 
iron ions in the blood serum increased in 2.2 times on 
the average in all studied groups, regardless of received 
treatment, as compared to the control group and ani-
mals without tumors. This opens up the opportunities 
for further development of the technology magnetic 
nanotherapy for oncology clinics. 
 
 
REFERENCES 
 
1. M. Johannsen, U. Gneveckow, B. Thiesen, K. Taymoorian, 
C.H. Cho, N. Waldöfner, R. Scholz, A. Jordan, 
S.A. Loening, P. Wust, Eur. Urol. 52, 6 (2007). 
2. V. Orel, A. Shevchenko, A. Romanov, M. Tselepi, 
T. Mitrelias, C.H.W. Barnes, A. Burlaka, S. Lukin, 
I. Shchepotin, Nanomed. Nanotech. Biol. Med. 11, 47 (2015). 
3. J.Y. Follézou, M. Bizon, Neoplasma 33, 2 (1986). 
 


Magnetic Nanotherapy by Magnetosensitive Nanocomplexes with Different Magnetic Properties of the Walker 256 Carcinosarcoma

Electronic Sumy State University Institutional Repository

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE  NANOMATERIALS: APPLICATIONS AND PROPERTIES Vol. 4 No 2, 02NABM01(3pp) (2015)   2304-1862/2015/4(2)02NABM01(3) 02NABM01-1  Magnetic Nanotherapy by Magnetosensitive Nanocomplexes with Different Magnetic  Properties of the Walker 256 Carcinosarcoma  V.E. Orel1,*, A.D. Shevchenko2, V.O. Melnik1, O.Yu. Rykhalskyi1, A.V. Romanov1  1 National Cancer Institute, 33/43 Lomonosov Str., 03022, Kyiv, Ukraine 2 G.V. Kurdyumov Institute for Metal Physics, N.A.S. of Ukraine, 36 Academican Vernadsky Boulevard, UA- 03680 Kyiv-142, Ukraine  (Received 03 April 2015; revised manuscript received 23 July 2015; published online 25 August 2015)  The magnetic nanocomplex (MNC) comprised of Fe3O4 nanoparticles (NP) and doxorubicin (DOXO) with a saturation magnetic moment ms  10.5 emu/g during magnetic nanotherapy initiated greater antitumor  effect than MNC with ms  8.55 emu/g. After treatment of Walker 256 tumor the concentration of iron ions in the blood serum of animals with tumor increased and did not depend on the magnetic properties of MNC.   Keywords: Nanoparticles, Carcinosarcoma Walker 256, Iron Ions, Blood Serum, Magnetic Nanotherapy.   PACS numbers: 75.75. + a, 87.85. – d                                                             *v-orel@voliacable.com 1. INTRODUCTION  Magnetic nanoparticles (NP) hyperthermia appears well-suited as an effective tumor therapy: (1) the con-centration of NP in the tumor is both sufficiently high and significantly higher than in surrounding, normal tissue, (2) the use of NP, which can combine both ther-apeutic and diagnostic capabilities in one dose, has the potential to lead toward personalized oncology and bet-ter outcomes for patients, (3) the particles possess a high enough specific absorption rate. However, there are a number of limitations of magnetic NP hyper-thermia such as: (1) therapy by NP in the temperature range 43–70 °C can be accompanied by the formation of drug resistance due to the induction of heat shock pro-teins, (2) the temperature above 45 C may shut down tumor tissue perfusion, and (3) targeted therapy with magnetic NP is often not suitable for disseminated and abdominal tumors [1]. To overcome the above problems, we have developed a new technology of magnetic nanotherapeutics based on multiple modes of actions, such as mild hyperther-mia less 40 C [2]. In this paper we studied an influence of magneto-sensitive nanocomplexes (MNC) with different magnet-ic properties on the iron distribution in serum of ani-mals with Walker 256 carcinosarcoma under magnetic nanotherapy.  2. MATERIAL AND METHODS  As a parts or drug components the NP of iron oxide Fe3O4 in diameter < 50 nm (Sigma-Aldrich) and do-xorubicin (DOXO) (Pfizer, Italy) have been used. There were two types of synthesized samples MNC with dif-ferent magnetic properties when exposed to 5 mT con-stant magnetic field (СMF) (MNС1) and 8 mT СMF (MNС2). Also, the effects of 5 mT and 8 mT CMF on the samples of iron oxide (labeled accordingly Fe3O4-1, Fe3O4-2) and DOXO (labeled accordingly DOXO1 and DOXO2) were examined during magneto-mechano-chemical activation. The study of antitumor activity of DOXO, MNC, CMF and electromagnetic irradiation (EI) was conduct-ed on 60 rats females weighing (175 ± 14) g from vivar-ium National Cancer Institute. Transplantation of tu-mor cells Walker 256 carcinosarcoma was performed by administration to the rat right thigh 20% cell suspen-sion in a volume of 0.4 ml in the medium 199. DOXO were injected into animals at a dose of 1.5 mg/kg, MNC: DOXO at a dose of 1.5 mg/kg, Fe3O4 at a dose of 3 mg/kg. Drug administration was carried out in the tail vein of animals in a volume of 0.3 ml 0.9% NaCl solu-tion. Treatment started 3 days after tumor transplan-tation and was performed five times every other day. Local EI of tumor animals was performed by experi-mental prototype device “Magnetotherm” (Radmir, Ukraine) with magnetic-dipole applicator that included needle localizer and neodymium permanent magnet with a maximum CMF magnetic induction of 0.4 T at a distance of 8 mm from the end of the dipoles and EMF frequency of 40 MHz with output power of 75 W. Tem-perature control was carried by fiber-optic thermometer TM-4 (Radmir, Ukraine). Intratumoral temperature did not exceed 38 C. The magnetic properties were studied by magne-tometery using a “Vibrating Magnetometer 7404 VSM” (Lake Shore Cryotronics, Inc., USA) with magnetic fields up to 13 kOe. The magnetometer’s sensitivity is 10-7 emu, and that allowed measurements of magnetic moment of samples weighing milligrams to be per-formed. The mass was determined by an electronic mi-crobalance AB135-S/FACT with auto-identification (Mettler Toledo, Switzerland) which has a sensitivity of 10-5 g. Colorimetric analysis with the Ferrozine (Ortho-Clinical Diagnostics, UK) has been used for the meas-urement of serum iron concentration by Vitros 250 (USA) chemistry analyzer.  V.E. OREL, A.D. SHEVCHENKO ET AL. PROC. NAP 4, 02NABM01 (2015)   02NABM01-2 3. RESULTS AND DISCUSSION  The effect of MNC with different magnetic proper-ties on the nonlinear kinetics of Walker 256 carcinosar- coma growth from the 3rd to the 15th day after tumor transplantation is shown at Table 1. According to the obtained data, a statistically significant difference was observed for anticancer effects of MNC1 as compared to MNC2 both without the influence CMF and EMF, and after combined action of CMF and EMF. The MNC an-titumor effect was evaluated on the base of the inhibi-tion coefficient of tumor growth. The MNC2 antitumor effect was 16.5 and 19% higher in the first and second case, respectively, as compared to MNC1.  Table 1 – An influence of MNC with different magnetic prop-erties on the growth kinetics of Walker 256 carcinosarcoma after irradiation by CMF and EMF  N Animal Group Parameter Growth Factor , day-1 Braking Ratio , Relative Units 1 Control (without treatment) 0.43±0.01 1.00 2 Conventional DOXO 0.32±0.01 1.35 3 MNC 1 0.40±0.01 1.09 4 MNC 2 0.33±0.02 1.31 5 MNC 1 + CMF + EI 0.34±0.01 1.27 6 MNC 2 + CMF + EI 0.28±0.02§ 1.56 , , , § Statistically significant difference from 1, 2, 3, and 5 animal group, respectively, р < 0.05  When compared antitumor effect of MNC with con-ventional DOXO it should be noted that only in the 6th group of animals after joint action of MNС2 with СMF and EMF the braking ratio of tumor growth was in-creased by 15.5%. In further observation of changing in tumor size till 22 days after transplantation the trend for the breaking ratio remaining unchanged in spite of the fact that some animals with large tumor size in 1–4 groups were died. An analysis of hysteresis loops (Fig. 1, Table 2) indi-cates that the samples of MNC, Fe3O4, DOXO1 and DOXO2 were soft ferromagnets. Conventional DOXO has diamagnetic properties. Greater saturation mag-netic moment and the magnetic hysteresis loop area had MNC and DOXO samples synthesised or activated in nanoreactor with CMF induction of 8 mT. The coer-cive force in this case decreased. For Fe3O4-2 unlike MNC2 an increase in coercivity and reduction of mag-netic hysteresis loop were reported as compared with Fe3O4-1. Iron ions are acceptor of electromagnetic field. Magnetic nanotherapy can influence the reaction yields of iron ions between the tumor and the blood. To un-derstand the distribution of the iron in the tumor and blood serum after magnetic nanotherapy we have ana-lyzed the concentration of iron and iron-containing pro-teins in the serum blood of animals. In Table 3 an in-fluence of MNC with different magnetic properties on iron concentration in blood serum of animals with Walker 256 carcinosarcoma after irradiation by CMF and EMF on the 15th day after tumor transplantation is shown. The content of iron ions in the blood serum increased in 2.2 times on the average in all studied groups, regardless of received treatment, as compared to the control group and animals without tumors. A comparison obtained experimental results and published in the paper [3] confirms the previously known tendency to increase of the iron content in blood serum under the influence of chemotherapy of malig-nant tumors. According to the above cited authors this is due to the effect of hemolysis, assuming that 60% of iron in the body is found in hemoglobin.     a     b     c  Fig. 1 – Hysteresis loops. MNC (а), Fe3O4 (b) and DOXO (c) at 38 С. Н – magnetic field; m – magnetic moment. В – CMF induction during magneto-mechano-chemical synthesis in nanoreactor: 1 – MNC1 (B = 5 mT), 2 – MNC2 (B = 8 mT)    ANTITUMOR EFFECT AND THE DISTRIBUTION … PROC. NAP 4, 02NABM01 (2015)   02NABM01-3 Table 2 – Magnetic properties of the samples at 38 С  Examined object MNC1 MNC2 Fe3O4-1 Fe3O4-2 DOXO1* DOXO2 CMF induction during magneto-mechano-chemical activation or synthe-sis in nanoreactor, mT 5 8 5 8 5 8 Magnetic hysteresis loops Saturation magnetic moment ms, emu/g 8.55 10.5 31.8 41.6 0.05 0.37 Coercive field Hc, Oe 13.1 4.4 15.2 18.62 30.1 24.8 Area of hysteresis loop, erg/g 136.3 1135.4 5613.3 1657 17 36.7 *Conventional DOXO is diamagnetic, magnetic moment m = – 1.18 emu/g at 3000 Oe   Table 3 – An influence of MNC with different magnetic prop-erties on the concentration of iron in blood serum of animals with Walker 256 carcinosarcoma after irradiation by CMF and EMF  N Animal group Iron, μmol/l 1 Control (without treatment) 15.4±1.2 2 Conventional DOXO 38.4±2.6 3 MNC 1 31.0±3.9 4 MNC 2 38.2±3.4 5 MNC 1 + CMF + EI 34.1±3.1 6 MNC 2 + CMF + EI 35.3±4.6 7 Animals without tumor 16.7±0.77§₤ , , , , §, ₤ Statistically significant difference from 1, 2, 3, 4, 5, and 6 animal group, respectively, р < 0.05  Thus, it is possible to state that the use of magnetic nanotherapy technology opens up the opportunities for further implementation in clinical practice the effect of the controlled local toxicity based on the selection of optimal magnetic properties of MNC.  4. CONCLUSION  Magnetic nanotherapy by the MNC comprised of Fe3O4 NP and DOXO with saturation magnetic mo-ment ms=10.5 emu/g and hysteresis loop area of 1135.4 erg/g has initiated greater growth inhibition factor of Walker-256 carcinosarcoma on 23% as compared to the MNC with lesser saturation magnetic moment ms=8.55 emu/g and hysteresis loop area of 136.3 erg/g. The con-tent of free iron complexes was minimal in Walker-256 carcinosarcoma after magnetic nanotherapy by MNC with a large hysteresis loop area. The concentration of iron ions in the blood serum increased in 2.2 times on the average in all studied groups, regardless of received treatment, as compared to the control group and ani-mals without tumors. This opens up the opportunities for further development of the technology magnetic nanotherapy for oncology clinics.   REFERENCES  1. M. Johannsen, U. Gneveckow, B. Thiesen, K. Taymoorian, C.H. Cho, N. Waldöfner, R. Scholz, A. Jordan, S.A. Loening, P. Wust, Eur. Urol. 52, 6 (2007). 2. V. Orel, A. Shevchenko, A. Romanov, M. Tselepi, T. Mitrelias, C.H.W. Barnes, A. Burlaka, S. Lukin, I. Shchepotin, Nanomed. Nanotech. Biol. Med. 11, 47 (2015). 3. J.Y. Follézou, M. Bizon, Neoplasma 33, 2 (1986).  

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Magnetic Nanotherapy by Magnetosensitive Nanocomplexes with Different Magnetic Properties of the Walker 256 Carcinosarcoma

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