slides
Enzymatic Hydrolysis of Beef Tallow in a Biphasic Organic-Aqueous System
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Abstract
Hydrolysis of beef tallow by soluble and immobilized lipase from Pseudomonas sp.
in a biphasic organic-aqueous reaction system was investigated. The addition of an
appropriate organic solvent to the reaction system stimulated the hydrolysis of beef
tallow by lipase strongly. Among the organic solvents tested,isooctane was found to be
the most suitable solvent for this purpose. The proportion of isooctane and phosphate
buffer in the reaction mixture was best at the ratio of 1:4. Under this experimental
condition,the reaction rate in a biphasic isooctane-aqueous buffer system was greatly
enhanced compared to that in the organic-solvent-free system,and the percentage of
hydrolysis reached about 40~65%