Multinucleate transfer cells induced in coleus roots by the root-knot nematode, Meloidogyne arenaria

Abstract

The occurrence and position of wall protuberances in giant cells induced in coleus roots by the root-knot nematodeMeloidogyne arenaria is described, and the structure and function of giant cells is compared with that of syncytia induced by cyst-nematodes. Extensive protuberance development occurs on walls of giant cells adjacent to xylem vessels. Protuberances are less well developed next to sieve elements, and almost absent next to parenchyma cells. On walls between giant cells they occur on both sides or only one side. The formation of protuberances indicates that giant cells are multinucleate transfer cells. The position of protuberances marks the wall area where solutes enter the cell. Solutes are obtained from xylem and phloem elements, and the position of protuberances at the junction between giant cells and vascular elements indicates an extensive flow of solutes along cell walls. The observations support the hypothesis that wall protuberances form as a result of selective solute flow across the plasmalemma. No cell wall dissolution was observed, although wall gaps may occur between giant cells as a result of breakage during rapid cell expansion