Growth Inhibition of Escherichia coli by Overexpression of Rat Perchloric Acid-soluble Protein

Abstract

Perchloric acid-soluble protein (PSP) and its highly homologous proteins are conserved among plants, archaea, bacteria, and eucarya. Although several functions of PSP have been elucidated in various species, the main role of PSP remains unclear. We previously demonstrated that PSP suppressed cell proliferation in mammalian cell lines. However, to the best of our knowledge, there are no reports on its function in other species. In this study, we investigated cell growth suppression of E. coli by rat PSP (rPSP). rPSP-His, GST-rPSP, or a GST expression vector were transformed into E. coli DH5α (DE3) cells, and expressions of recombinants were induced by IPTG. All recombinant expressions were detected in the presence of > 0.05 mM IPTG. The growth of transformed E. coli cells was suppressed by rPSP-His and GST-rPSP. Suppression ability of rPSP-His was stronger than that of GST-rPSP