Objectives: The aim of this study is to investigate the relation among TNF-a -6
concentration/expression with placental tissue infarction and apoptosis between PE-E and non
PE-E.
Material and method: Research subjects were 35 subjects, composed of 17 non PE-E and 18
PE-E parturient that delivery or hospitalized on Department of OBGYN Dr. Kariadi General
Hospital Semarang. The concentration/expression of TNF- -6 were measured from vein blood
sample and placental tissue by ELISA and immunohystochemistry (IHC) method. Apoptosis area
was measured by IHC with Acridine orange staining.
Results: The average of infarction on normal subjects was 12.5%, however, on PE-E was 35.3%
(p=0.001). The average of apoptosis on normal subjects was 32.3 %, however, on PE-E subjects
were 71.0% (p=0.001). The average of placenta TNFpg/
mL, on PE-E was 2.0 pg/mL (p<0.001). The average of serum TNFnormal
subjects was 2.3 pg/mL, on PE-E was 2.8 pg/mL (p<0.001). The average of placenta IL-
6 concentration of normal subjects was 0.6 pg/mL, on PE-E was 1.3 pg/mL (p<0.001). The
average of serum IL-6 concentration of normal subjects was 1.4 pg/mL, on PE-E was 2.0
pg/mL (p<0.001). There were a significant correlation among TNF-a -6 serum and placenta
with apoptosis of placenta.
Conclusion: Proinflammatory cytokines concentration on serum and placenta extract of subjects
with PE-E were significantly higher than non PE-E. There was a significant correlation between
proinflammatory cytokines concentration on serum or placenta with apoptosis of placental tissue.
Keywords: TNF-a , IL-6, infarct, apoptosis, placenta, preeclampsia, eclampsia
