For Assembly 20 of the Candida albicans genome, the sequence of each of the eight chromosomes was determined, revealing new insights into gene family creation and dispersion, subtelomere organization, and chromosome evolution

Berriman, Matthew

Chibana, Hiroji

Cuomo, Christine

Dignard, Daniel

Grindle, Suzanne

Hogues, Hervé

Magee, BB

Magee, PT

Martchenko, Mikhail

Nantel, André

Rast, Timothy J

Scherer, Stewart

van het Hoog, Marco

Whiteway, Malcolm

English

PubMed

Marco van het Hoog

Timothy J Rast

Mikhail Martchenko

Suzanne Grindle

Daniel Dignard

Hervé Hogues

Christine Cuomo

Matthew Berriman

Stewart Scherer

BB Magee

Malcolm Whiteway

Hiroji Chibana

André Nantel

PT Magee

Springer - Publisher Connector

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes

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The 10.9x genomic sequence of Candida albicans, the most important human fungal pathogen, was published in 2004. Assembly 19 consisted of 412 supercontigs, of which 266 were a haploid set, since this fungus is diploid and contains an extensive degree of heterozygosity but lacks a complete sexual cycle. However, sequences of specific chromosomes were not determined. RESULTS: Supercontigs from Assembly 19 (183, representing 98.4% of the sequence) were assigned to individual chromosomes purified by pulse-field gel electrophoresis and hybridized to DNA microarrays. Nine Assembly 19 supercontigs were found to contain markers from two different chromosomes. Assembly 21 contains the sequence of each of the eight chromosomes and was determined using a synteny analysis with preliminary versions of the Candida dubliniensis genome assembly, bioinformatics, a sequence tagged site (STS) map of overlapping fosmid clones, and an optical map. The orientation and order of the contigs on each chromosome, repeat regions too large to be covered by a sequence run, such as the ribosomal DNA cluster and the major repeat sequence, and telomere placement were determined using the STS map. Sequence gaps were closed by PCR and sequencing of the products. The overall assembly was compared to an optical map; this identified some misassembled contigs and gave a size estimate for each chromosome. CONCLUSION: Assembly 21 reveals an ancient chromosome fusion, a number of small internal duplications followed by inversions, and a subtelomeric arrangement, including a new gene family, the TLO genes. Correlations of position with relatedness of gene families imply a novel method of dispersion. The sequence of the individual chromosomes of C. albicans raises interesting biological questions about gene family creation and dispersion, subtelomere organization, and chromosome evolutionNRC publication: Ye

Rast, Timothy J.

Hogues, Herv\ue9

Magee, B. B.

Nantel, Andr\ue9

Magee, P. T .

NRC Publications Archive

Assembly of the  genome into sixteen supercontigs aligned on the eight chromosomes

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Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes

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