Routine environmental monitoring is the prime necessity to maintain a Specific Pathogen Free (SPF) facility. Monitoring the animals & its environment is important to control the contamination and outbreak of disease. Appropriate documentation, labeling, handling, time, source, collection, transport, processing & storage of samples are important to keep the sample integrity. The sampling process needs to be well coordinated with both the vivarium and lab team. Proper disposal should be maintained throughout the sampling process. The animal holding rooms are monitored for both the microbial contamination and keep the husbandry, washing area of the facility free from contamination