Durzan, Don J

Havel, Ladislav

Santerre, Anne

BMC Plant Biology

English

PubMed

Don J Durzan

Anne Santerre

Ladislav Havel

Springer

Diploid parthenogenesis and 'early embryo' development in Norway spruce cell suspensions

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BMC Plant Biology
Open AccessMeeting abstract
Diploid parthenogenesis and 'early embryo' development in 
Norway spruce cell suspensions
Don J Durzan*1, Anne Santerre1 and Ladislav Havel2
Address: 1Department of Plant Sciences, University of California, One Shields Ave. Davis, CA 95616, USA and 2Department of Botany and Plant 
Physiology, Mendel University of Agriculture and Forestry, 613-00 Brno, Zemedelsko1, Czech Republic
Email: Don J Durzan* - djdurzan@ucdavis.edu
* Corresponding author    
In diploid parthenogenesis, 'proembryos' developed from
binucleate egg-equivalents containing an egg nucleus and
an apoptotic ventral canal nucleus. As the axial tier of
'early embryos' formed, the cell regulatory proteins in pro-
embryonal cells were ubiquitinated and turned over as
suspensors differentiated. Axial tier formation was
blocked at high levels of chlorsulfuron, an inhibitor of
acetolactate synthase. This enzyme is required for the bio-
synthesis of branched-chain amino acids, which are espe-
cially abundant in ubiquitin. The block of acetolactate
synthase led to the accumulation of free ∀-amino-n-
butyrate. The overall behavior of branched-chain amino
acids revealed rigid and linear relations over all chlorsul-
furon levels. The proliferating cell nuclear antigen
(PCNA), required for DNA synthesis, was detected in rap-
idly cycling proembryonal cells. PCNA appeared to serve
as a factor maintaining the cell replication typical of rap-
idly growing early embryos. Less than 0.01% of nuclei
reacted with epitopes to anti-p53 and anti-p21 that are
commonly associated with cell cycle arrest and DNA dam-
age. The cleavage sites of early embryos involved apopto-
sis and contributed to their multiplication. Chlorsulfuron
contributed to aborted axial tier development due to dis-
rupted patterns for the ubiquitination of cell regulatory
proteins as revealed by changes in the soluble amino
acids.
from Cell Biology of Nitric Oxide and Cell Death in Plants
Yalta, Ukraine, 8–11 September 2004
Published: 31 May 2005
BMC Plant Biology 2005, 5(Suppl 1):S13 doi:10.1186/1471-2229-5-S1-S13
<supplement> <title> <p>Cell Biology of Nitric Oxide and Cell Death in Plants</p> </title> <note>Meeting abstracts</note> </supplement>


Havel Ladislav

Santerre Anne

Durzan Don J

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Diploid parthenogenesis and 'early embryo' development in Norway spruce cell suspensions

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