Li, Bing

Li, Hengguang

Wang, Jinsong

Wang, Lai-Xi

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Retrovirology
Open AccessOral presentation
Toward a glycopeptide-based HIV-1 vaccine
Lai-Xi Wang*, Bing Li, Hengguang Li and Jinsong Wang
Address: Institute of Human Virology, University of Maryland Biotechnology Institute, Baltimore, Maryland, 21201, USA
Email: Lai-Xi Wang* - wangx@umbi.umd.edu
* Corresponding author    
A major difficulty in HIV-1 vaccine development is to
identify conserved neutralizing epitopes. We hypothesize
that conserved HIV-1 glycopeptides, a partial structure of
the envelope glycoproteins, represent new types of neu-
tralizing epitopes for several reasons: 1) certain HIV-1
glycopeptides are highly conserved and are well accessi-
ble; 2) a novel glycan cluster has been identified as the
neutralizing epitope for the broadly neutralizing antibody
2G12, indicating N-glycan itself could serve as target of
vaccine; and 3) the interactions of the carbohydrate and
peptide epitope may generate new conformational
epitope that could not be achieved by peptide or carbohy-
drate alone. To test this hypothesis, we have been explor-
ing HIV-1 V3 domain glycopeptides that combine the
conserved N-glycans as an essential epitope in immuno-
gen design. Toward this end, we have developed a novel
chemoenzymatic method for constructing large homoge-
neous HIV-1 V3 glycopeptides that are hitherto unavaila-
ble. Preliminary studies have revealed that the
glycosylation affects the global conformation of the V3
domain and can protect the V3 domain against protease
digestion. The binding of the synthetic HIV-1 glycopep-
tides with neutralizing antibodies (2G12, 447-52D) and
other V3-specific antibodies is in progress and the impli-
cations of the results for effective immunogen design will
be discussed.
from 2006 International Meeting of The Institute of Human Virology
Baltimore, USA. 17–21 November, 2006
Published: 21 December 2006
Retrovirology 2006, 3(Suppl 1):S75 doi:10.1186/1742-4690-3-S1-S75
<supplement> <title> <p>2006 International Meeting of The Institute of Human Virology</p> </title> <note>Meeting abstracts. A single PDF containing all abstracts in this Supplement is available <a href=" http://www.biomedcentral.com/content/files/pdf/1742-4690-3-S1-full.pdf ">here</a></note> <url>http://www.biomedcentral.com/content/pdf/1742-4690-3-S1-info.pdf</url></supplement>
© 2006 Wang et al; licensee BioMed Central Ltd. 


Toward a glycopeptide-based HIV-1 vaccine

Wang Jinsong

Li Hengguang

Li Bing

Wang Lai-Xi

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Retrovirology

Lai-Xi Wang

Bing Li

Hengguang Li

Jinsong Wang

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Toward a glycopeptide-based HIV-1 vaccine

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