Castañeyra-Perdomo, Agustín

Castañeyra-Ruiz, Agustin

Castañeyra-Ruiz, Leandro

Fernández-Rodríguez, Paloma

Garcia, Dionisio M

Gonzalez-Marrero, Ibrahim

Gonzalez-Toledo, Juan M

Ma Carmona-Calero, Emilia

Perez-Gonzalez, Herminia

Troyano, Juan M

English

PubMed

Troyano Juan M

Castañeyra-Ruiz Agustin

Castañeyra-Ruiz Leandro

Gonzalez-Toledo Juan M

Perez-Gonzalez Herminia

Fernández-Rodríguez Paloma

Gonzalez-Marrero Ibrahim

Ma Carmona-Calero Emilia

Garcia Dionisio M

Castañeyra-Perdomo Agustín

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Cerebrospinal Fluid Research

Dopamine β-hydroxylase and p73 expression during aging and ventricular dilation in the cerebrospinal fluid and circumventricular organs of SHR rats

Emilia Ma Carmona-Calero

Ibrahim Gonzalez-Marrero

Paloma Fernández-Rodríguez

Herminia Perez-Gonzalez

Juan M Gonzalez-Toledo

Leandro Castañeyra-Ruiz

Agustin Castañeyra-Ruiz

Juan M Troyano

Dionisio M Garcia

Agustín Castañeyra-Perdomo

Springer - Publisher Connector

BioMed CentralCerebrospinal Fluid Research
ssOpen AccePoster presentation without discussion
Dopamine β-hydroxylase and p73 expression during aging and 
ventricular dilation in the cerebrospinal fluid and circumventricular 
organs of SHR rats
Emilia Ma Carmona-Calero*1,2, Ibrahim Gonzalez-Marrero1,2, 
Paloma Fernández-Rodríguez2, Herminia Perez-Gonzalez1, 
Juan M Gonzalez-Toledo1, Leandro Castañeyra-Ruiz2, Agustin Castañeyra-
Ruiz1, Juan M Troyano1, Dionisio M Garcia1 and Agustín Castañeyra-
Perdomo1,2
Address: 1Departamento de Anatomía, Facultad de Medicina, Universidad de La Laguna, Tenerife, Islas Canarias, Spain and 2Departamento de 
Biotecnología, Instituto de Investigación y Ciencias de Puerto del Rosario 35600, Fuerteventura, Spain
Email: Emilia Ma Carmona-Calero* - ecarmona@ull.es
* Corresponding author    
Background
It has been reported that spontaneously hypertensive rats
(SHR) show ventricular dilation variations and changes in
CSF proteins. The organum vasculosum of the lamina ter-
minalis (OVLT) and the subfornical organ (SFO) are cir-
cumventricular organs located in the third ventricle,
which are rich in neuropeptides such as angiotensin II and
catecholamines. The SFO has connections with the brain
regions involved in the central regulation of blood pres-
sure and cardiovascular function. p73 is present in devel-
oping neurons as a truncated isoform whose levels
decrease dramatically during sympathetic neuron apopto-
sis after nerve growth factor (NGF) withdrawal. Therefore,
p73 is necessary for the survival and long-term mainte-
nance of CNS neurons. The purpose of the present work is
to study the dopamine beta-hydroxylase (DBH) and p73
expression in the SCO, the OVLT and the SFO and their
variations in ventricular dilatation and arterial hyperten-
sion.
Materials and methods
Brains and cerebrospinal fluid (CSF) from control Wistar-
Kyoto rats (WKY) and spontaneously hydrocephalus rats
(SHR) were used. The paraffin section containing the
SCO, OVLT and SFO were immunohistochemically proc-
essed with anti-DBH and anti-p73. DBH and p73 band
were identified in the CSF and circumventricular organ
extract by western blot
Results
The SHR of 6 and 12 months of age showed a greater
increase in ventricle size than those of normal sized WKY
rats. In the electrophoretic study, we detected the presence
of five protein bands: 141, 117, 48, 43, 39 kDa in the CSF
of the SHR rats, that were scarcely present in the CSF of the
WKY rats. We also found that DBH was lower in the SFO
of the hypertensive rats than in the WKY rats, and p73
expression was higher in the SFO of the hypertensive rats
than in the WKY rats. While p73 was heavily expressed in
the SCO and OVLT of the WKY rats this was scarce in the
SCO and OVLT of the SHR rats. The SFO extract bands
were also marked with anti-DBH and anti-p73 and the
from 50th Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida
Cambridge, UK. 30 August – 2 September 2006
Published: 21 December 2006
Cerebrospinal Fluid Research 2006, 3(Suppl 1):S61 doi:10.1186/1743-8454-3-S1-S61
<supplement> <title> <p>50th Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida</p> </title> <note>Meeting abstracts – A single PDF containing all abstracts in this supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1743-8454-3-S1-full.pdf ">here</a>.</note> </supplement>
© 2006 Ma Carmona-Calero et al; licensee BioMed Central Ltd. Page 1 of 2
(page number not for citation purposes)
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intensity of the reaction was higher in SHR than in the
WKY rats.
Conclusion
The present results and the fact that the deltaNp73 is nec-
essary for the survival of sympathetic neurons, would
indicate that p73 is an essential survival protein in CNS
catecholaminergic neurons, therefore there could be a
relationship between p73, DBH and these cerebral centres
involved in cardiovascular regulation.Page 2 of 2
(page number not for citation purposes)


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Dopamine β-hydroxylase and p73 expression during aging and ventricular dilation in the cerebrospinal fluid and circumventricular organs of SHR rats

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