Angeletti, RH

Basu, J

Duttagupta, C

Nieves, E

Romney, SL

Vermund, SH

English

PubMed

J Basu

C Duttagupta

RH Angeletti

E Nieves

SH Vermund

SL Romney

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BioMed Central
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Retrovirology
Open AccessPoster presentation
HIV-associated cellular proteins in human cervicovaginal lavage 
samples from HIV-negative women
J Basu*1, C Duttagupta1,2, RH Angeletti3, E Nieves3, SH Vermund4 and 
SL Romney1
Address: 1Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA, 2Department of 
Biotechnology, Institute of Technology and Marine Engineering, West Bengel, India, 3Department of Development and Molecular Biology, Albert 
Einstein College of Medicine, Bronx, New York, 10461, USA and 4Institute of Global Health and Department of Pediatrics, Vanderbilt University, 
School of Medicine, Nashville, Tennessee 37232, USA
* Corresponding author    
In a series of 20 women, HIV gag and env glycoproteins
were detected by Mass Spectroscopy (MS) and confirmed
by Western blot (WB) analysis in four women whose
serum ELISA tests were HIV antibody negative. The pres-
ence of prostate-specific antigen in the CVL samples were
analyzed by WB to determine whether the proteins might
be from male deposition, and were found to be negative.
We have no explanation at present for the finding of HIV
proteins in these HIV seronegative women. MS data also
identified the presence of albumin complexed with myris-
tic acid in the CVL samples. Using immunoaffinity chro-
matography and Microcon YM-50 filters, the albumin in
the CVL samples was removed and the fractions were ana-
lyzed by SDS-PAGE and WB analysis using a monoclonal
anti-HIV-1 p24 antibody. HIV-1 p24 positive bands were
seen only in albumin-conjugated fractions. N-myristoyla-
tion of the HIV-1 gag protein is necessary for efficient env
protein transportation to the cell surface. Mutations
blocking this modification lead to a failure of extracellular
viral particle production. Moreover, maleylated human
serum albumin is reported to inhibit HIV-1 infection and
syncytia formation between HIV-1 and target cell in vitro.
The association of HIV-1 p24 with albumin in CVL sam-
ples is currently being explored. Our data suggests that cel-
lular albumin could be exploited as cellular target for HIV
inhibition and in the development of new drugs.
from 2006 International Meeting of The Institute of Human Virology
Baltimore, USA. 17–21 November, 2006
Published: 21 December 2006
Retrovirology 2006, 3(Suppl 1):P7 doi:10.1186/1742-4690-3-S1-P7
<supplement> <title> <p>2006 International Meeting of The Institute of Human Virology</p> </title> <note>Meeting abstracts. A single PDF containing all abstracts in this Supplement is available <a href=" http://www.biomedcentral.com/content/files/pdf/1742-4690-3-S1-full.pdf ">here</a></note> <url>http://www.biomedcentral.com/content/pdf/1742-4690-3-S1-info.pdf</url></supplement>
© 2006 Basu et al; licensee BioMed Central Ltd. 


HIV-associated cellular proteins in human cervicovaginal lavage samples from HIV-negative women

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HIV-associated cellular proteins in human cervicovaginal lavage samples from HIV-negative women

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