Dilernia, DA

Losso, M

Lourta, L

Salomon, H

Retrovirology

English

PubMed

DA Dilernia

L Lourta

M Losso

H Salomon

Springer - Publisher Connector

BioMed Central
Page 1 of 1
(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
Differential HLA-dependent HIV evolution among subtypes
DA Dilernia*1, L Lourta2, M Losso3 and H Salomon1
Address: 1National Reference Center for AIDS, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina, 2Hospital Ramos Mejia, 
Buenos Aires, Argentina and 3Nexo Asociación Civil, Buenos Aires, Argentina
* Corresponding author    
Introduction
Population HLA-dependent evolution has been docu-
mented over the principal HIV proteins. Effect of subtype
variability is uncertain. In Argentina, B subtype and
recombinant BF variants circulate in equal proportions.
Methods
Blood samples were collected from 94 drug-naive HIV
infected individuals. DNA and RNA extraction were per-
formed from PBMC and plasma, respectively. HLA-A and
B genes were genotyped by PCR-SSOP. Pol and vpu genes
were amplified by RT-PCR and sequenced. Aminoacidic
positions were codified as 0 = "equal state" (to consensus
sequence) and 1 = "variated state" (polymorphism). Phy-
logenetic analyses were performed by BootScanning. Sta-
tistical analysis was performed by ODDS-ratio/power
calculations, Fischer exact test, Logistic regression and ran-
domization tests. Potential new epitopes and affinities
with HLA were assessed by using BIMAS software.
Results
We found 95 polymorphisms associated with different
HLA alleles. 44 out of them were inside predicted epitopes
for the associated HLA allele. 13 out of them reduced the
affinity for the HLA allele in the "variated" state. 5 out of
them were associated with viral subtype.
Conclusion
Our results suggest that vpu could be undergoing adapta-
tion to the immune response at the population level, as
previously reported for pol gen. Associations with viral
subtype suggest that this evolution is different among sub-
types and that variants from subtype B and BF could
respond differently to both natural and vaccine-induced
immune response.
from 2006 International Meeting of The Institute of Human Virology
Baltimore, USA. 17–21 November, 2006
Published: 21 December 2006
Retrovirology 2006, 3(Suppl 1):P15 doi:10.1186/1742-4690-3-S1-P15
<supplement> <title> <p>2006 International Meeting of The Institute of Human Virology</p> </title> <note>Meeting abstracts. A single PDF containing all abstracts in this Supplement is available <a href=" http://www.biomedcentral.com/content/files/pdf/1742-4690-3-S1-full.pdf ">here</a></note> <url>http://www.biomedcentral.com/content/pdf/1742-4690-3-S1-info.pdf</url></supplement>
© 2006 Dilernia et al; licensee BioMed Central Ltd. 


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