Repeated cultivation of crop plants is the reason for the depletion of nutrients in an agricultural land. Therefore, modern procedures of agriculture cascaded with the addition of organic and inorganic fertilizers, the use of insecticides and pesticides, the addition of proper water, etc. Various inorganic molecules are used as fertilizers. However, the use of organic manures is also in practice. They have many roles such as improving soil porosity, air holding capacity, water holding capacity, structure, texture, etc. Agricultural scientists suggest using organic molecules for many reasons. Bio-fertilizers of many kinds are used by farmers of all nations. However, these fertilizers are unable to cause tremendous effects on the growth and development of crop plants, even though these fertilizers have cumulative effects. The present work focuses on the use of bio-molecules as bio-fertilizer. To make these molecules, an amylolytic bacterium was isolated and partially identified based on microscopic observations and biochemical tests. The optimum pH, temperature, substrate concentration, etc. were studied. The optimum pH and temperature for the growth of the isolate were pH 7.0 and 37.0°C, respectively. However, the organism grows even in 60.0°C. The organism uses four commonly available natural substrates as carbon source. Among these, potato starch is the most conveniently utilized by the organism. The amy gene of the strain was cloned using a vector. It expressed a high amount of amylase (data is not shown). The recombinant organism was used to make bio-molecules. It was grown in the presence of various natural substrates and enzymatic activities, and other associated studies were also carried out. The experimental results obtained in this study showed that the recombinant organism can be utilized to make a huge amount of bio-molecules. It will be a unique fertilizer for future generations
