HTself is a web-based bioinformatics tool designed to deal with the classification of differential gene expression for low replication microarray studies. It is based on a statistical test that uses self-self experiments to derive intensity-dependent cutoffs. The method was previously described in Vêncio et al, (DNA Res. 12: 211- e 214, 2005). In this work we consider an extension of HTself by calculating p-values instead of using a fixed credibility level α. As before, the statistic used to compute single spots p-values is obtained from the gaussian Kernel Density Estimator method applied to self-self data. Different spots corresponding to the same biological gene (replicas) give rise to a set of independent p-values which can be combined by well known statistical methods. The combined p-value can be used to decide whether a gene can be considered differentially expressed or not. HTself2 is a new version of HTself that uses the idea of p-values combination. It was implemented as a user-friendly desktop application to help laboratories without a bioinformatics infrastructure
