Two fungal strains were isolated and tentatively identified as Penicillium VY and Aspergillus VY. All the isolated species show the maximum production on third day in a liquid culture media. The pH optimum was found to be 10.0 for Penicillium VY and 11.0 for Aspergillus VY. The temperature optima were 50ºC in both the cases. The enzyme produced by Penicillium VY was found to be stable in the pH range 3.0-7.0 and 3.0–6.0 in case of Aspergillus VY. The enzyme does not loose activity up to 40º C in case of Penicillium VY and 40ºC in case of Aspergillus VY if exposed for 1 h.

