Novel sensitive colorimetric assay for visual detection of solid-phase bound amines

Abstract

A new simple and efficient method for the detection of incomplete coupling reactions during solid-phase peptide synthesis is decribed. Using p-nitrophenyl ester 1 (NF31), free amino groups can be visually detected on the resin by direct coloring of the beads. A specific feature of the assay resides in the possibility of detection of sterically hindered primary amines