Alfeld, Matthias

Boesenberg, Ulrike

Claussen, Thorsten

Czyzycki, Mateusz

De Samber, Björn

Falkenberg, Gerald

Garrevoet, Jan

Roeb, Marion

Schroeder, Walter H

Vergucht, Eva

Vincze, Laszlo

English

Garrevoet, JanUGent00008052778080200091718997257211009513DABCE2-F0EE-11E1-A9DE-61C894A0A6B4

De Samber, BjörnUGent0020020595868020000723809786039398100000-0002-6759-4195F87EB214-F0ED-11E1-A9DE-61C894A0A6B4

Vergucht, EvaUGent00006015323580200110603897768482035001CFAC4C-F0EE-11E1-A9DE-61C894A0A6B4

Vincze, LaszloWE068010018449760000-0002-1184-9822F7139566-F0ED-11E1-A9DE-61C894A0A6B4

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Cryogenic analysis of frozen hydrated biological tissue at the Hard X-ray Micro-Probe Beamline at PETRA III

Ghent University Academic Bibliography

 Cryogenic Analysis of Frozen Hydrated Biological Tissue at the Hard X-ray Micro-Probe Beamline at PETRA III J. Garrevoet1, W. H. Schroeder1,2, B. DeSamber1,3, T. Claußen1, E. Vergucht3, U. Boesenberg1, M. Alfeld1, M. Czyzycki1,4, M. Roeb2, L. Vincze3, G. Falkenberg1 1Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany, 2Forschungszentrum Jülich, IBG-2, 52425 Jülich, Germany 3X-ray Microspectroscopy and Imaging Research Group, Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, B-9000 Gent, Belgium,  4AGH University of Science & Technology, Faculty of Physics & Applied Computer Science, Al. A. Mickiewicza 30, 30-059 Krakow, Poland Introduction and Objectives A robust cryogenic workflow was developed at beamline P06 to minimise structural and chemical artefacts during sample preparation as well as beam damage during analysis. Shock frozen hydrated biological and medical tissue samples up to a diameter of 2 mm can be analysed. The developed instrument consists of a vacuum chamber (ca. 10-8 mbar), two cooling systems, one for rapid cooling at startup and one vibration free long term cooling system maintining the temperature between -120 °C and -150 °C, an ultrathin polymer window XRF detector, an absorption imaging detector (PCO 4000), and a cryogenic vacuum transfer system. The presented system allows besides 2D and 3D elemental imaging also absorption and phase contrast tomography on the (sub-)micrometer scale.  Results and Discussion  Recent results will be presented, demonstrating the capabilities regarding absorption/phase tomography and fluorescence imaging on frozen-hydrated biological samples. Analysis of a rice leaf shows the ability of the presented instrument to obtain high resolution 3D images, while the Si elemental image shows the high sensitivity and resolution of the XRF imaging system. Conclusions Combining a full cryogenic workflow with a cryogenic measurement instrument allows for high resolution structural and high sensitivity elemental analysis without the need of any chemical sample preparation steps, preserving the original state as much as possible.    Figure 1 (Top) A crop of a 3D CT of a shock frozen hydrated rice leaf. (Center) Front absorption view (Bottom) A 2D XRF Si image, showing Si inclusions in the epidermis of a rice leaf. 

https://biblio.ugent.be/publication/6958176/file/6958180.pdf

Cryogenic analysis of frozen hydrated biological tissue at the Hard X-ray Micro-Probe Beamline at PETRA III

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Ghent University Academic Bibliography