Bachert, Claus

Cardell, Lars-Olaf

Fokkens, Wytske

Förster, Ulrike

Hellings, Peter

Kowalski, Marek

Mullol, Joaquim

Olze, Heidi

Tomassen, Peter

Toskala, Elina

English

Ghent University Academic Bibliography

 47GA2LEN sinusitis cohort study: chronic rhinosinusitis with nasal polyps — differentiation based onchemokine patternOlze, Heidi1; Förster, Ulrike1; Cardell, Lars-Olaf2; Fokkens, Wytske3; Kowalski, Marek4; Hellings, Peter5; Mullol,Joaquim6; Toskala, Elina7; Tomassen, Peter8; Bachert, Claus91Charité, Otorhinolaryngology, Berlin, Germany; 2Karolinska Institutet, Division of ENT diseases, CLINTEC,Stockholm, Sweden; 3Academic Medical Centre, Amsterdam, Otorhinolaryngology, Amsterdam, Netherlands; 4Medical University of Lodz, Department of Immunology, Rheumatology and Allergy,, Lodz, Poland; 5UniversityHospitals Leuven, Otorhinolaryngology, Head and Neck Surgery, Leuven, Belgium; 6IDIBAPS, Clinical andExperimental Respiratory Immunoallergy, Barcelona, Spain; 7Helsinki University Hospital, Finnish Institute ofOccupational Health, Helsinki, Finland; 8Ghent University, Upper Airways Research Laboratory, Ghent, Belgium; 9Ghent University, Upper Airways Research Laboratory, Otorhinolaryngology, Ghent, BelgiumBackground: Chronic Rhinosinusitis (CRS) with (CRSwNP) and without nasal polyps (CRSsNP) are consideredheterogeneous entities of CRS. Studying inflammatory cells and their mediators in clearly defined subgroups (EP3OScriteria) may lead to a better differentiation of CRS. The aim of present study was 1. to differentiate those subgroupsby means of the EP3OS criteria 2. to characterize the chemokine pattern in nasal tissue of CRSs/wNP and 3. toevaluate the prevalence of IL-5 positivity and its impact on chemokine profile.Method: There were 98 patients included in our prospective multicenter, case-control study; CRSwNP n=28,CRSsNP n=45 and 25 controls undergoing septoplasty or conchotomy. Nasal tissue homogenates were analyzed byELISA for a range of chemokines: eotaxin, TARC, PARC, ENA-78, IP-10, MIP-1alpha, MCP-3 and for IL-5.Result: In nasal tissue of CRSwNP patients we observed significantly elevated concentration of eotaxin, TARC, PARC,ENA-78 and IP-10, as compared to controls. Patients with CRSsNP had only elevated concentration of TARC, ascompared to the control group. CRSwNP was characterized by increased concentration in tissue homogenates ofeotaxin, PARC, TARC and IL-5, but not of MIP-1alpha and MCP-3 compared to CRSsNP. Nasal tissue of CRSsNPpatients contained significantly more of ENA-78 and IP-10 than CRSwNP patients. IL-5 was detectable 34.1% of theCRSsNP group and in 84.6% of CRSwNP samples. IL-5 positive CRSsNP samples had a significant higher chemokineconcentration for eotaxin, MIP-1alpha, PARC and TARC than IL-5- negative tissue. In IL-5 positive NP PARC wasincreased significantly compared with IL-5 negative NP.Conclusion: Defining the chemokine pattern may help to characterize specific CRS entities. Cluster analysis insubgroups can lead to further understanding and classification of CRS. IL-5 appears to be a promising diagnosticmarker for further typing of CRS.

GA2LEN sinusitis cohort study: chronic Rhinosinusitis with nasal polyps: differentiation based on chemokine pattern

https://biblio.ugent.be/publication/1354025/file/1860618

GA2LEN sinusitis cohort study: chronic Rhinosinusitis with nasal polyps: differentiation based on chemokine pattern

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