Cox, Eric

Melkebeek, Vesna

Vandamme, Katrien

Vervaet, Chris

English

Ghent University Academic Bibliography

The	  adjuvant	  effect	  of	  Gantrez®AN	  nanoparticles	  on	  oral	  vaccination	  of	  pigs	  and	  mice	  with	  F4	  fimbriae	  is	  strongly	  influenced	  by	  polymer	  degradation.	  	  Melkebeek,	  Vesna1;	  Vandamme,	  Katrien2;	  Vervaet,	  Chris2;	  Cox,	  Eric1	  	  1.	  Laboratory	  of	  Immunology,	  Ghent	  University,	  Merelbele,	  Belgium.	  2.	  Laboratory	  of	  Pharmaceutical	  Technology,	  Ghent	  University,	  Gent,	  Belgium.	  	  We	  analysed	  the	  adjuvant	  effect	  of	  Gantrez	  nanoparticles	  NP	  on	  oral	  immunisation	  of	  pigs	  and	  mice	  with	  F4	  fimbriae.	  The	  animals	  were	  vaccinated	  with	  F4,	  F4	  encapsulated	  in	  Gantrez	  NP,	  called	  gF4	  NP,	  or	  F4	  +	  empty	  Gantrez	  NP,	  called	  F4	  +	  gNP,	  and	  intragastrically	  infected	  with	  F4+	  ETEC.	  In	  pigs,	  a	  clear	  F4	  specific	  serum	  IgA	  and	  IgG	  response	  following	  vaccination	  could	  only	  be	  observed	  in	  the	  F4+g	  NP	  group.	  Also	  in	  mice,	  the	  strongest	  response	  could	  be	  seen	  in	  the	  F4	  +	  g	  NP	  group.	  In	  contrast	  to	  the	  results	  in	  pigs	  however,	  encapsulation	  of	  F4	  in	  NP	  reduced	  the	  response.	  An	  important	  difference	  between	  mice	  and	  pigs	  is	  that	  pigs	  have	  an	  intestinal	  F4	  receptor,	  whereas	  mice	  don’t	  have	  this	  receptor.	  Taken	  together,	  in	  both	  mice	  and	  pigs,	  the	  best	  adjuvant	  effect	  was	  seen	  by	  adding	  empty	  NP	  to	  the	  fimbriae.	  These	  data	  suggested	  that	  functional	  groups	  at	  the	  surface	  of	  the	  NP	  are	  likely	  to	  play	  a	  significant	  role	  in	  the	  immunogenicity.	  To	  analyze	  if	  the	  adjuvant	  effect	  of	  the	  empty	  NP	  was	  sufficient	  to	  protect	  suckling	  pigs,	  the	  experiment	  was	  repeated	  6	  months	  later.	  However,	  the	  response	  in	  the	  F4	  +	  g	  NP	  group	  was	  not	  improved	  compared	  to	  the	  F4	  group.	  To	  explain	  the	  discrepancy	  between	  the	  studies,	  the	  polymer	  was	  characterized	  again	  and	  a	  second	  mice	  experiment	  was	  performed	  to	  analyze	  the	  influence	  of	  storage	  on	  the	  polymer	  properties.Changes	  in	  polymer	  weight	  and	  polymer	  weight	  distribution	  occurred.	  In	  addition,	  the	  adjuvant	  effect	  of	  empty	  NP	  on	  F4	  in	  mice	  was	  lost.	  Nevertheless,	  this	  could	  be	  restored	  by	  crosslinking	  the	  NP	  more	  strongly.	  	  

The adjuvant effect of Gantrez®AN nanoparticles on oral vaccination of pigs and mice with F4 fimbriae is strongly influenced by polymer degradation

https://biblio.ugent.be/publication/2020696/file/2020702.pdf

The adjuvant effect of Gantrez®AN nanoparticles on oral vaccination of pigs and mice with F4 fimbriae is strongly influenced by polymer degradation

Abstract

Similar works

Full text

Available Versions

Ghent University Academic Bibliography