De Muynck, Cassandra

LEROY, A

Soetaert, Wim

Vandamme, Erick

VERSTRAETEN, W

Undetermined

Ghent University Academic Bibliography

Presented at the Renewable Resources and Biorefineries Conference, Ghent, Belgium, 19-21 September 2005 Production and antifungal activity of reuterin  C. De Muynck1, A. Leroy1, W. Verstraeten1, F.Arnaut2, W. Soetaert1 and E. Vandamme1  1Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Gent, Belgium, e-mail: Cassandra.DeMuynck@UGent.be 2Puratos N.V., Industrialaan 25, B-1702 Groot-Bijgaarden, Belgium  Reuterin, which comprises β-hydroxypropionaldehyde and its hydrated and dimeric forms, is a small valuable compound that can be used in a myriad of applications. Because of its antimicrobial activity, reuterin can be applied as such in food preservation or produced in situ by the reuterin-producing strain, Lactobacillus reuteri, in probiotic formulas. Reuterin can also be used as a precursor for the synthesis of several chemicals such as acrolein, acrylic acid and 1,3-propanediol [1] and as an alternative to glutaraldehyde in the sterilization and fixation of prostheses derived from biological tissue [2]. Reuterin can be produced via traditional chemistry and by bacterial bioconversion. The bio-process is based on a simple and clean single enzymatic step, which converts the renewable and cheap resource glycerol with high yield into β-hydroxypropionaldehyde. We have studied this bioconversion by using viable cell suspensions of Lactobacillus reuteri and partially optimized the process for the production of pure reuterin.  OHOHOH- H2Oglyceroldehydratase+ co-enzyme B12OHOHglycerol β-hydroxypropionaldehyde  We have also assessed the antifungal activity of reuterin, which was found to inhibit the growth of a range of spoilage fungi (Penicillium, Aspergillus etc.) and also Bacillus sp. on an agar-plate assay with reuterin added to a flour-containing medium. Contrary to the well studied antibacterial activities, the antifungal metabolites of lactic acid bacteria remain far less explored [3]. Reuterin could act as a model bio-active molecule in this context.  [1] Vollenweider, S. & Lacroix, C. (2004). Applied Microbiology and Biotechnology 64, 16-27. [2] Chen, C., Sung, H., Liang, H. & Chang, W. (2002). Journal of Biomedical Materials Research 61, 360-369. [3] De Muynck, C., Leroy, A. De Maeseneire, S., Arnout F., Soetaert, W. & Vandamme, E. (2004). Microbiological Research 159, 339-346. 

Production and antifungal activity of reuterin

https://biblio.ugent.be/publication/322384/file/452526

Production and antifungal activity of reuterin

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Ghent University Academic Bibliography