Claerebout, Edwin

Cox, Eric

Devriendt, Bert

Geldhof, Peter

Geurden, Thomas

Grit, Grietje

Hope, J

Van Coppernolle, Stephanie

Vercruysse, Jozef

English

Ghent University Academic Bibliography

 Semi maturation of bovine monocyte-derived dendritic cells after incubation with Giardia duodenalis   G. H. Grit1, S. Van Coppernolle1, B. Devriendt1, T. Geurden1, J. Hope2, J. Vercruysse1, P. Geldhof1, E. Cox1, E. Claerebout1  1Department of Virology, Parasitology & Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium 2The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK  Giardia duodenalis is an important intestinal parasite in animals and humans. The role of dendritic cells in the immune response against G. duodenalis is poorly documented and has only been studied in the mouse, which is not a natural host for this parasite. In this study we addressed the effect of G. duodenalis trophozoites and excretion/secretion (ES) products on the expression of maturation markers and cytokine production by bovine monocyte-derived dendritic cells (MoDCs) in vitro. Moreover, the ability of stimulated MoDCs to take up antigen and to induce mononuclear cell (MC) proliferation was assessed.  Although none of the maturation markers CD40, CD80 and MHCII were up-regulated in MoDC cultures after stimulation with Giardia trophozoites or ES, a dose-dependent decrease of ovalbumin uptake was observed. IL-15 transcription was significantly increased after 24h of stimulation with Giardia trophozoites. Other cytokines were not significantly up- or down-regulated. MoDCs stimulated with Giardia trophozoites or ES induced a dose-dependent proliferation of allogenic γδ-T-cells and TCRαβ+ CD4+ and -CD8+ T-cells in vitro, compared to cultures with unstimulated MoDCs. Induction of a CD4+ T-cell response by Giardia-stimulated MoDCs was confirmed in an autologous lymphocyte proliferation assay, using MoDCs and PBMCs from calves artificially infected with G. duodenalis cysts. Our data show that G. duodenalis trophozoites induce a functional maturation of bovine MoDCs. Functionally active MoDCs, lacking the expression of co-stimulatory molecules are known as semi-mature DC. Although semi-mature DC can cause T-cell tolerance, MoDCs stimulated with G. duodenalis showed a significantly increased mRNA transcription level of IL-15, which can explain the activation and proliferation of the T-cell populations despite the lack of co-stimulatory molecules present on the cell surface. Proliferating CD4+ T-cells will be further characterised to investigate whether a regulatory T-cell response is induced by Giardia-stimulated MoDCs.  Acknowledgements:  G.H. Grit is funded by a PhD grant from IWT-Vlaanderen.  S. Van Coppernolle is funded by the EU FP7 project (Paravac) B. Devriendt is funded by a post-doc grant of Ghent University  

Semi maturation of bovine monocyte-derived dendritic cells after incubation with Giardia duodenalis

https://biblio.ugent.be/publication/4146632/file/4146652.pdf

Semi maturation of bovine monocyte-derived dendritic cells after incubation with Giardia duodenalis

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Ghent University Academic Bibliography