Berckmans, D

Chiers, Koen

Cox, Eric

De Waele, Tine

Duchateau, Luc

Gasthuys, Frank

Goddeeris, Bruno

Stuyven, Edith

English

Ghent University Academic Bibliography

Title:	  Prediction	  of	  disease	  outcome	  in	  an	  acute	  lung	  infection	  in	  pigs:	  promising	  role	  for	  IL-­‐6?	  T.	  De	  Waele	  1	  ,	  L.	  Duchateau	  3,	  K.	  Chiers6,	  E.	  Stuyven	  1	  ,F.	  Gasthuys	  4,	  D.	  Berckmans	  5	  ,E.	  Cox	  1,B.	  Goddeeris	  1,2	  1Laboratory	  of	  Immunology,	  Faculty	  of	  Veterinary	  Medicine,	  Ghent	  University,	  Salisburylaan	  133,	  B-­‐9820	  Merelbeke,	  Belgium	  2Department	  of	  Biosystems,	  Laboratory	  of	  Livestock	  Physiology	  and	  Immunology,	  Faculty	  of	  Bioscience	  Engineering,	  Kasteelpark	  Arenberg	  30,	  B-­‐3001	  Heverlee,	  Belgium	  3	  Department	  of	  Physiology	  and	  Biometry,	  Faculty	  of	  Veterinary	  Medicine,	  Ghent	  University,	  Salisburylaan	  133,	  B-­‐9820	  Merelbeke,	  Belgium	  4Department	  of	  Surgery	  and	  anaesthesiology	  of	  domestic	  animals,	  Faculty	  of	  Veterinary	  Medicine,	  Ghent	  University,	  Salisburylaan	  133,	  B-­‐9820	  Merelbeke,	  Belgium	  5Department	  of	  Biosystems,	  M3-­‐BIORES,	  Faculty	  of	  Bioscience	  Engineering,	  Kasteelpark	  Arenberg	  30,	  B-­‐3001	  Heverlee,	  Belgium	  6Laboratory	  of	  Pathology,	  Faculty	  of	  Veterinary	  Medicine,	  Ghent	  University,	  Salisburylaan	  133,	  B-­‐9820	  Merelbeke,	  Belgium	  The	  objective	  of	  this	  study	  was	  to	  look	  at	  serum	  concentrations	  of	  several	  cytokines	  and	  acute	  phase	  proteins	  at	  regular	  time-­‐points	  before	  and	  after	  infection,	  and	  based	  on	  the	  dynamics	  of	  these	  markers,	  	  to	  identify	  possible	  marker(s)	  for	  prediction	  of	  disease	  outcome	  in	  an	  acute	  and	  sometimes	  fatal	  lung	  infection	  in	  pigs.	  In	  this	  study,	  after	  socialization,	  acclimatization	  and	  catheterization,	  13	  pigs	  were	  inoculated	  endobronchially	  with	  5*106	  CFU	  of	  Actinobacillus	  	  pleuropneumoniae.	  Blood	  samples	  and	  clinical	  scores	  were	  obtained	  every	  	  2	  hours	  from	  4	  hours	  before	  infection	  until	  48	  hours	  after	  infection	  or	  until	  death/euthanasia.	  In	  these	  samples,	  serum	  concentrations	  of	  IL-­‐6,	  IL-­‐8,	  IL-­‐10,	  TNF-­‐α,	  Pig	  MAP,	  SAA,	  CRP	  and	  Haptoglobin	  were	  determined	  with	  standard	  ELISA	  methods.	  	  	  After	  48	  hours,	  5	  pigs	  survived	  and	  8	  pigs	  died	  within	  8-­‐34	  hours	  after	  inoculation.	  Survival	  analysis	  was	  used	  to	  evaluate	  whether	  the	  time-­‐varying	  IL-­‐6	  serum	  concentration	  was	  predictive	  for	  the	  status	  of	  the	  animal	  (acute	  versus	  non	  acute).	  IL-­‐6	  was	  predictive	  for	  the	  status	  of	  the	  animal	  2	  hours	  later,	  with	  high	  IL-­‐6	  serum	  concentrations	  corresponding	  to	  higher	  risk	  (hazard	  ratio=1.37;	  P=0.008).	  Out	  of	  these	  results,	  we	  can	  conclude	  that	  IL-­‐6	  could	  be	  a	  predictive	  marker	  for	  disease	  outcome,	  looking	  at	  the	  dynamics	  of	  this	  cytokine	  in	  pig	  serum.	  

Prediction of disease outcome in an acute lung infection in pigs: promising role for IL-­‐6?

https://biblio.ugent.be/publication/1178270/file/1178292

Prediction of disease outcome in an acute lung infection in pigs: promising role for IL-‐6?

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Ghent University Academic Bibliography

Prediction of disease outcome in an acute lung infection in pigs: promising role for IL-­‐6?

Abstract

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Full text

Available Versions

Ghent University Academic Bibliography

Prediction of disease outcome in an acute lung infection in pigs: promising role for IL-‐6?