Daminet, Sylvie

De Rooster, Hilde

Demeyere, Kristel

Maddens, Bert

Meyer, Evelyne

Smets, Pascale

English

Ghent University Academic Bibliography

URINARY IMMUNOGLOBULIN  G,  C-REACTIVE PROTEIN AND RETINOL BINDING  PROTEIN AS  CANDIDATE  EARLY BIOMARKERS  FOR  RENAL DYSFUNCTION IN DOGS WITH PYOMETRAB.  E.J.   Maddens  1  , S. Daminet2, P. Smets2, K. Demeyere1, H. de Rooster2, E. Meyer1.1Department  of  Pharmacology,  Toxicology,  Biochemistry  and  Organ  Physiology 2Department of Medicine and Clinical Biology of Small Animals, both Faculty of Veterinary Medicine, Ghent University, Belgium.Renal  dysfunction  in  dogs  has  been  associated  with  pyometra.  Nevertheless,  the mechanism and type of renal injury remain controversial. Routine measurement of serum urea nitrogen and creatinine detects  renal  injury only in a late irreversible stage  and does  not  indicate  the  localisation  of  the  damage.  We hypothesize  that urinary immunoglobulin  G (uIgG),  C-reactive  protein (uCRP) and retinol  binding protein (uRBP) may serve as superior renal biomarkers. Both IgG and CRP are high molecular weight proteins indicating glomerular lesions, whereas RBP may serve as a tubular damage marker.In this study, 14 dogs with Escherichia coli (E. coli) pyometra (P) without concurrent other diseases were included. Age-matched clinically healthy bitches (H) served as controls (n=14). At ovariohysterectomy (P, H) or ovariectomy (H) blood, urine and uterine  swabs  were  taken.  Serum  biochemical  analysis,  CBC,  urinanalysis  and uterine  bacteriological  culture  were  performed.  Commercial  canine  ELISAs  were validated  and  used  to  quantify  uIgG  and  uCRP;  uRBP  concentrations  were determined  with  a  human  RBP  ELISA  kit  validated  for  use  in  the  dog.  All concentrations were related to urinary creatinine concentration (uC) and expressed as ratios.In P dogs, uIgG/C (196 ± 45.2 mg/g) and uCRP/C (706 ± 298 µg/g) (mean ± SEM) ratios were significantly increased compared to those in H bitches (2.1 ± 0.4 mg/g respectively 0.1 ± 0.1 µg/g) (P<0.01). Furthermore, uRBP/C ratios were significantly higher in P (43.7 ± 10.1 µg/g) than in H dogs (16.7 ± 5.5 µg/g) (P<0.05). A positive correlation at the 0.05 level was found between the urinary concentrations of all three proteins. Urinary total protein/C ratios (uTP/C) were significantly higher in P (0.77 ± 0.19) than in H dogs (0.16 ± 0.06) (P<0.05). A highly significant positive correlation was found between uTP/C and uIgG/C (R = 0.79) or uCRP/C (R = 0.72), but not between uTP/C and uRBP/C.Concentrations of uIgG and uCRP, the latter for the first time examined in the dog, are  strongly elevated  in  P  bitches  indicating  glomerular  dysfunction  immediately after  E. coli pyometra.  The  milder  increase  in  uRBP  is  suggestive  for  tubular dysfunction,  possible  secondary  to  the  proteinuria  related  to  immune-mediated glomerulonephritis.  We suggest  that the observed increases in the candidate renal markers  uIgG,  uCRP and  uRBP might  be  indicative  of  renal  dysfunction  at  the glomerular and tubular level, associated with E. coli pyometra.

Urinary immunoglobulin G, C-reactive protein and retinol-binding protein as candidate early biomarkers for renal dysfunction in dogs with pyometra

https://biblio.ugent.be/publication/593863/file/593864.pdf

Urinary immunoglobulin G, C-reactive protein and retinol-binding protein as candidate early biomarkers for renal dysfunction in dogs with pyometra

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Ghent University Academic Bibliography