De Graef, Jessie

Claerebout, Edwin

Vercruysse, Jozef

von Samson-Himmelstjerna, G

Skuce, P

Mitreva, M

Geldhof, Peter

English

Ghent University Academic Bibliography

Abstract 2  Jessie De Graef   Ghent University 	   1/1	  Analysis of ABC-transporters in Cooperia oncophora  De Graef J.1, Claerebout E.1, Vercruysse J.1, von Samson-Himmelstjerna G.2, Skuce P.3, Mitreva M.4, Geldhof P.1 1Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University, Belgium. 2Institute for Parasitology, University of Veterinary Medicine Hannover, Germany 3Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, Scotland, United Kingdom. 4Department of Genetics, The Genome Center, Washington University School of Medicine, St. Louis, Missouri, United States of America. Apart from target site alteration, anthelmintic resistance may also be mediated by mechanisms that alter the drug concentration at the sites of action. Members of the ATP-binding cassette transporter family (P-glycoproteins, Haf-transporters, Multidrug Resistant proteins) may fulfil this job. Over-expression of ABC-transporter genes can contribute to drug resistance in parasites. To identify ABC transporters in C. oncophora, we analyzed the transcriptome dataset of larval and adult life stages. We could identify homologues of Pgp-2, Pgp-3, Pgp-9, Pgp-11, Pgp-12, Pgp-16, Haf-2, Haf-3, Haf-4, Haf-9, Mrp-4 and Mrp-7. By reverse-transcriptase PCR we determined that all these genes are equally expressed in all life stages (Egg, L1, L2, L3, L4, Male, Female) of C. oncophora. Quantitative real-time PCR’s were subsequently performed to compare the mRNA transcription levels of all identified ABC’s in adult worms of an ivermectin (IVM)-susceptible and an IVM-resistant isolate collected from the field. The data indicated that none of the genes had an altered transcription pattern between the two isolates. However, after exposure to IVM, Pgp-11 transcription levels in resistant worms were significant upregulated compared to non-exposed worms. Whether Pgp-11 is actually involved in the resistance mechanism or whether the upregulation of this gene is a result of a stress response in the worms is still unclear.    

Analysis of ABC-transporters in Cooperia oncophora

https://biblio.ugent.be/publication/2007039/file/2007040.pdf

Analysis of ABC-transporters in Cooperia oncophora

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Ghent University Academic Bibliography