Beer, Martin

Herden, Christiane

Hoffmann, Donata

Nobach, Daniel*

Schlottau, Kore

English

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Poster Presentations New and Re-Emerging Zoonotic Diseases 134 N04  Histological lesions and virus detection in rats, bank voles and mice infected with variegated squirrel bornavirus 1 (VSBV-1)  Daniel Nobach1, Kore Schlottau2, Donata Hoffmann2, Martin Beer2 and Christiane Herden1,3  1Justus-Liebig-Universität, Institut für Veterinär-Pathologie, Gießen, Germany 2Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany 3Center of Mind, Brain and Behavior, Justus-Liebig-University Gießen, Gießen, Germany  Keywords: animal model, bornavirus, rodents, encephalitis  Variegated squirrel bornavirus 1 (VSBV-1) was identified as cause of human lethal encephalitis. Since 2015, it has been shown to be present in various exotic squirrel populations in zoos and private husbandries.  For the development of small animal models representing either the reservoir (squirrel) or the accidental host (humans), neonatal and adult rats (n=94), bank voles (n=55) and mice (n=96) were infected with VSBV-1 by different routes (intracerebral, intranasal or subcutan). After three / five months, animals were examined for macroscopic and histological lesions. Virus distribution was analyzed by immunohistochemistry. Macroscopic lesions were found in none of the animals. Some rats (n=3) and bank voles (n=2) displayed a lymphohistiocytic encephalitis with perivascular cuffs and reactive microglia. Viral antigen was present in neurons and astrocytes in the hippocampus, cerebral cortex, thalamus and brain stem in intracerebrally infected rats (n=3) and bank voles (n=3). Additionally, in one subcutaneously infected bank vole neurons and astrocytes of spinal ganglia and spinal cord harboured viral antigen. No virus antigen was found in peripheral organs. These results indicate different courses of infection depending on the species, the age and the route of infection. The presence of viral antigen in the CNS reflects a strict neurotropism like in accidental host, but the duration of the infection could influence the results and has to be taken into consideration.    

Histological lesions and virus detection in rats, bank voles and mice infected with
variegated squirrel bornavirus 1 (VSBV-1)

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Histological lesions and virus detection in rats, bank voles and mice infected with variegated squirrel bornavirus 1 (VSBV-1)

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