Predicting drug penetration across the blood-brain barrier: comparison of different stationary phases for immobilized artificial membrane liquid chromatography
Several in vitro methods have been tested for their ability to predict drug penetration across the blood-brain barrier into the central nervous system. In this article, the performance of three stationary phases for immobilized artificial membrane (IAM) liquid chromatographic approaches were compared on a set of 49 compounds. IAM liquid chromatography measurements were performed with Dulbecco’s phosphate-buffered saline and methanol as organic modifier in the mobile phase. Transport across the blood-brain barrier (log BB) was predicted using computed descriptor data and the retention factor of all compounds. All data were correlated with experimental log BB values and the relative performance of the approaches was studied. The IAM.PC.DD2 column proved to be the best suited for prediction of log BB values, although all three columns performed very good
