Dubruel, Peter

Giol, Elena Diana

Kirkpartick, C James

Unger, Ron E

Van Vlierberghe, Sandra

English

Ghent University Academic Bibliography

 Surface-modified PET for cardiovascular applications: preliminary biocompatibility studies   E.D. Giol 1, R. E. Unger2, S. Van Vlierberghe1,3, C.J. Kirkpatrick2 and P Dubruel3,a  1Polymer Chemistry and Biomaterials Research (PBM) Group, Ghent University, Krijgslaan 281 (S4 bis), Ghent, Belgium; 2 REPAIR LAB, Institute of Pathology, University Medical Center of the , Johannes Gutenberg University Mainz, Langenbeckstrasse 1, Mainz, Germany; 3 Brussels Photonics Team, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, Belgium  email: peter.dubruel@ugent.be (correspounding author) Keywords: poly(ethylene terephthalate), cardiovascular, surface modification, dopamine, HUVEC Abstract.  Half of the annual human deaths worldwide are due to cardiovascular diseases [1]. Currently, synthetic grafts play a vital role in medicine, as implant demands exceed natural available sources and thus alternative solutions are needed. Poly(ethylene terephthalate) or PET is extensively used as a synthetic graft material on account of its excellent bulk properties (high mechanical and thermal properties) and chemical inertness. A major drawback is its poor surface properties that directly influence biological performance. Herein, we present a nature-inspired surface modification strategy suitable for fine-tuning surface properties of PET grafts. Materials & Methods. A two-step surface modification strategy was applied to PET (see Fig 1.). A dopamine-based coating procedure was used to facilitate the subsequent covalent immobilization of gelatin. In parallel, physisorbed surfaces were obtained by dip-coating.  A surface characterization was performed using SCA, AFM and XPS, coupled with radiolabelling. Also, preliminary haemocompatibility and in vitro assays were applied.   Fig. 1: Schematic representation of the general surface modification strategy.  Results & Discussion. Current surface modification strategies consist either of complex multi-step and time-consuming steps or the use of compounds (e.g. glutaraldehyde) that can raise toxicity issues [3]. Therefore, most synthetic grafts are generally modified by a physical means, neglecting coating stability issues. To circumvent these problems, we have developed an easy aqueous-based                                           TioxidationcleaningTiOH OH OHOHTiX X XX polymer immobilisationTifunctionalisation                                 –                         -    -                         TioxidationcleaningTiOH OH OHOHTiX X XX polymer immobilisationTifunctionalisation    TioxidationcleaningTiOH OH OHOHTiX X XX polymer immobilisationTifunctionalisation                        method to surface-modify PET. A dopamine coating was applied to facilitate the covalent immobilization of gelatine type B as target biocompatible protein [2].  A comparative study between physically and chemically modified PET was pursued. Surface modification and I125 gelatine radiolabelling techniques showed clear differences. Covalent immobilization led to a more homogeneous and stable coating. Interestingly, minimal platelet activation was also observed, while HUVEC cell adhesion tests underlined the importance of a stable coating.  Conclusion. Stability and toxicity issues were avoided by using a 100 % organic solvent-free method to surface modify PET for cardiovascular applications. The importance of a covalent-based coating was shown to play an essential role in both the haemocompatibility and the in vitro cell behaviour.  References [1] European Cardiovascular Disease Statistics (2005 edition), University of Oxford. [2] S. Van Vlierberghe, E. Vanderleyden, V. Boterberg, P. Dubruel, Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization, Polymers, 3 (1, 2011) 114-130. [3] Y. Noishiki, X. H. Ma, Y. Yamane, S. Satoh, T. Okoshi, K. Takahashi, Y. Iwai, T. Kosuge, Y. Ichikawa, I. Yamazaki, M. Mo, Succinylated collagen crosslinked by thermal treatment for coating vascular prostheses,  Artificial Organs  22(8, 1998) 672-680.  ACKNOWLEDGEMENT: The authors would like to thank the FWO for granting Elena Diana Giol a long-term abroad research fellowship to Germany and Ghent University for the financial support. This work was co-financed by ERDF and made possible by INTERREG IV A 2Seas programme.  

Surface-modified PET for cardiovascular applications : preliminary biocompatibility studies

https://biblio.ugent.be/publication/5743846/file/5743853

Surface-modified PET for cardiovascular applications : preliminary biocompatibility studies

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