Baert, Kim

Cox, Eric

Devriendt, Bert

Goddeeris, Bruno

Luo, Yu

English

Ghent University Academic Bibliography

ETEC	  colonization	  factors	  modulate	  the	  antigen	  presentation	  function	  of	  porcine	  intestinal	  mononuclear	  phagocytes	  	  Bert	  DEVRIENDT,	  Kim	  BAERT,	  Yu	  LUO,	  Bruno	  M.	  GODDEERIS,	  Eric	  COX	  	  	  Laboratory	   of	   Immunology,	   Faculty	   of	   Veterinary	   Medicine,	   University	   of	   Ghent,	   9820	  Merelbeke,	  Belgium	  	  Enterotoxigenic	   E.	   coli	   (ETEC)	   are	   still	   a	   major	   cause	   of	   traveller’s	   diarrhea	   and	   intestinal	  infections	   in	   children	   in	   less	   affluent	   countries.	   In	   addition,	   ETEC	   infections	   lead	   to	  postweaning	   diarrhea	   in	   piglets	   and	   result	   in	   significant	   economic	   losses	   in	   swine	  production.	  This	   intestinal	  pathogen	  displays	  colonization	  factors	  or	   fimbriae	  on	   its	  surface	  enabling	  the	  colonization	  of	  the	  small	  intestinal	  epithelia.	  In	  swine,	  F4	  and	  F18	  fimbriae	  are	  frequently	   associated	   with	   ETEC-­‐induced	   diarrhea.	   As	   opposed	   to	   F4	   fimbriae,	   oral	  immunisation	  with	  F18	  fimbriae	  fails	  to	  protect	  animals	  from	  a	  challenge	  infection.	  Besides	  structural	  differences	  and	  a	  different	  uptake	  mechanism	  by	  the	  intestinal	  epithelium	  of	  F18	  (M-­‐cell	  dependent	   transport)	  and	  F4	   fimbriae	   (M-­‐cell	  and	  enterocyt-­‐dependent	   transport),	  these	  fimbriae	  could	  differently	  modulate	  the	  function	  of	  intestinal	  phagocytes.	  Indeed,	  F18	  fimbriae	   drastically	   diminished	   the	   antigen	   presentation	   capacity	   of	   mononuclear	  phagocytes	  (MHCII+SIRPαhi)	  isolated	  from	  the	  small	  intestinal	  lamina	  propria.	  As	  F18	  fimbriae	  bind	   glycosphingolipids	   and	   presumably	   disrupt	   lipid	   raft	   formation,	   the	   impaired	   antigen	  presentation	  ability	  of	  the	  intestinal	  phagocytes	  could	  result	  from	  a	  F18	  fimbriae-­‐mediated	  deformation	  of	  the	  immunological	  synapse.	  These	  results	  could	  accelerate	  the	  development	  of	  an	  improved	  F18+	  ETEC	  vaccine	  and	  hint	  at	  novel	  immune	  evasive	  mechanisms	  employed	  by	  bacterial	  pathogens.	  

ETEC colonization factors modulate the antigen presentation function of porcine intestinal mononuclear phagocytes

https://biblio.ugent.be/publication/4146621/file/4146631.pdf

ETEC colonization factors modulate the antigen presentation function of porcine intestinal mononuclear phagocytes

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Ghent University Academic Bibliography