Britton, Robert A

Crabbé, Aurélie

De Weirdt, Rosemarie

Lacroix, Christophe

Nickerson, Cheryl A

Roos, Stefan

Sarker, Shameema

Van de Wiele, Tom

van Pijkeren, Jan Peter

Vollenweider, Sabine

English

Ghent University Academic Bibliography

TITLE: GLYCEROL SUPPLEMENTATION ENHANCES LACTOBACILLUS REUTERI’S PROTECTIVE EFFECT AGAINST SALMONELLA TYPHIMURIUM INFECTION IN THE COLON  AUTHORS: Rosemarie De Weirdt1, Aurélie Crabbé2, Stefan Roos3, Sabine Vollenweider5, Christophe Lacroix4, Shameema Sarker2, Tom Van de Wiele1, Cheryl CA Nickerson2  AFFILIATION:  1 Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium 2 Center for Infectious Diseases and Vaccinology - The Biodesign Institute, Arizona State University, PO Box 875401, Tempe AZ 85287-5401, USA 3 Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, SE-750 07 Uppsala, Sweden 4 Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland 5 Flavour Science & Technology, Natural Flavour Ingredients, Givaudan Schweiz AG - Ueberlandstrasse 138 CH- 8600 Dübendorf - Switzerland  KEYWORDS: Lactobacillus reuteri, glycerol, colon epithelium, host-microbe, Salmonella  ABSTRACT: Lactobacillus reuteri’s probiotic effects have been speculated to partly depend on its capacity to convert glycerol, commonly present in feces as food or pharmaceutical residual, to the antimicrobial reuterin. In this study we investigated the potential of this process to protect human intestinal cells against infection by the enteric pathogen Salmonella Typhimurium. We used a highly differentiated model of human colonic epithelium, which was previously found to reflect key characteristics of the in vivo Salmonella infection process. This model was infected with S. Typhimurium (1) in the presence of an established L. reuteri community without or with glycerol, or (2) under continuous exposure to the sterile L. reuteri ferment without and with reuterin (produced from glycerol) or pure reuterin. Using this model, glycerol supplementation was found to significantly enhance the protective effect of an established L. reuteri population against the early stages of Salmonella infection. We further demonstrated that continuous exposure to the reuterin-containing glycerol ferment of L. reuteri or pure reuterin caused a significant reduction of 1-2 log units in Salmonella adherence, invasion and intracellular survival. Exposure to the L. reuteri ferment without glycerol significantly stimulated intracellular Salmonella growth with 1 log-unit. These data demonstrate the importance of glycerol and reuterin for L. reuteri’s effects on pathogen survival. However, long-term exposure (24h) to pure reuterin or the reuterin-containing ferment induced a complete loss of cell-cell contact within the epithelial structure and compromised cell viability.  Collectively, these results shed light on a biological role for reuterin in inhibiting intestinal Salmonella infection and support the combined application of glycerol and L. reuteri in reducing intestinal Salmonella infection, dependent upon future in vivo studies of reuterin on intestinal health. To our knowledge, this is the first report of the effect of reuterin on the enteric infection process in any mammalian cell type.     

Glycerol supplementation enhances Lactobacillus reuteri's protective effect against Salmonella Typhimurium infection in the colon

https://biblio.ugent.be/publication/2007297/file/2007301.pdf

Glycerol supplementation enhances Lactobacillus reuteri's protective effect against Salmonella Typhimurium infection in the colon

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Ghent University Academic Bibliography