Ducatelle, Richard

Flahou, Bram

Haesebrouck, Freddy

Pasmans, Frank

Smet, Annemieke

Van Deun, Kim

Vermoote, Miet

English

Ghent University Academic Bibliography

In Vitro Antimicrobial Susceptibility of Helicobacter suis 1  2 Miet Vermoote*, Frank Pasmans, Kim Van Deun, Bram Flahou, Annemieke Smet, Richard 3 Ducatelle, and Freddy Haesebrouck 4  5 Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, 6 Merelbeke, Belgium 7  8 Helicobacter (H.) suis is a porcine gastric pathogen, which is also considered to be of 9 zoonotic importance. No in vitro antimicrobial susceptibility data are available for this very 10 fastidious micro-organism that only grows on a biphasic culture medium. Therefore, a 11 combined agar and broth dilution method followed by a standardized H. suis specific 12 quantitative real-time PCR (qPCR) assay was used to analyse the activity of 9 antimicrobial 13 agents: ampicillin, ceftiofur, clarithromycin, enrofloxacin, gentamicin, lincomycin, 14 metronidazole, tetracycline, and tylosine. After 48 hours microaerobic incubation, minimal 15 inhibitory concentrations (MICs) were determined for 9 H. suis isolates by software-assisted 16 calculation of bacterial growth. One, one and three isolates displayed acquired resistance to 17 enrofloxacin, lincomycin and ceftiofur, respectively, as indicated by a bimodal distribution of 18 the MICs. The MICs of ampicillin displayed a monomodal distribution, but with tailing 19 toward the higher MIC values for 5 isolates, possibly indicating reduced susceptibility. For 20 the other antimicrobial agents a monomodal distribution of MIC values was observed, 21 indicating absence of acquired resistance, although in 7 isolates, MICs of metronidazole were 22 equal or higher than the breakpoint proposed for H. pylori. The significance of the findings 23 presented here for treatment of humans or animals infected with H. suis needs further 24 investigation.  25  26 

In vitro antimicrobial susceptibility of Helicobacter suis

https://biblio.ugent.be/publication/1148041/file/1199909

In vitro antimicrobial susceptibility of Helicobacter suis

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Ghent University Academic Bibliography