MICROSATELLITE DIVERSITY IN BOS TAURUS, EQUUS CABALLUS AND GALLUS DOMESTICUS BREEDS REARED IN UKRAINE

Abstract

This study is dedicated to the comparative analysis of the main parameters of microsatellite variability in the populations of animals from different taxa (Bos taurus, Equus caballus, and Gallus domesticus) of different breeds, reared in Ukraine. To investigate microsatellite variability, the following SSR-markers were used: for Bos taurus – TGLA126, TGLA122, INRA023, ETH003, ETH225, BM1824, TGLA227, BM2113, ETH10 and SPS115; for Equus caballus – HTG04, HMS06, AHT04, ASB23, HTG07, HTG06, CA425, VHL20, HMS03, HMS07 and ASB17; for Gallus domesticus – ADL0268, ADL0278, MCW0248, LEI0094 and MCW0216. The results of analyzing the parameter of the average number of alleles per locus (A) were used to determine their least amount in Gallus domesticus (6.56) and the highest one – in Equus caballus (10.76). The observed data are in agreement with the standardization procedure results, based on the rarefaction analysis on the level of 25 animals for each specific species of animals. The highest values of the total genetic diversity (uHe) were notable for Bos taurus (0.835), and the lowest ones – for Gallus domesticus (0.690). These results were confirmed by the Shannon’s index values (1.940 for Bos taurus, 1.886 for Equus caballus and 1.420 for Gallus domesticus) as well as by the number of effective alleles (6.166; 5.614 and 3.848, respectively). The value of genetic subdivision (differentiation) according to Fst values fluctuated depending on the taxon and amounted to 0.119 for Gallus domesticus; 0.043 for Equus caballus and 0.03 for Bos taurus. Genetic differentiation between the populations, evaluated by the analysis of molecular variance (AMOVA), was in the range from 3 to 14 % for different taxa. Mikrosatelitska raznolikost pri pasmah Bos taurus, Equus caballus in Gallus domesticus, vzrejenih v Ukrajini Izvleček: Raziskava je bila posvečena primerjalni analizi glavnih parametrov variabilnosti mikrosatelitov v populacijah živali različnih taksonov (Bos taurus, Equus caballus in Gallus domesticus) različnih pasem, ki se vzrejajo v Ukrajini. Za raziskovanje mikrosatelitske variabilnosti so bili uporabljeni naslednji SSR-označevalci: za Bos taurus – TGLA126, TGLA122, INRA023, ETH003, ETH225, BM1824, TGLA227, BM2113, ETH10 in SPS115; za Equus caballus – HTG04, HMS06, AHT04, ASB23, HTG07, HTG06, CA425, VHL20, HMS03, HMS07 in ASB17; za Gallus domesticus – ADL0268, ADL0278, MCW0248, LEI0094 in MCW0216. Na podlagi rezultatov analize parametra povprečnega števila alelov na lokus (A) smo njihovo najmanjšo količino določili pri Gallus domesticus (6,56) in največjo pri Equus caballus (10,76). Ugotovljeni podatki so v skladu z rezultati postopka standardizacije, ki temelji na analizi redkosti na ravni 25 živali za vsako posamezno živalsko vrsto. Najvišje vrednosti skupne genetske raznolikosti (uHe) so bile opazne za Bos taurus (0,835), najnižje pa za Gallus domesticus (0,690). Te rezultate so potrdili vrednosti Shannonovega indeksa (1,940 za Bos taurus, 1,886 za Equus caballus in 1,420 za Gallus domesticus) ter število učinkovitih alelov (6,166; 5,614 in 3,848). Vrednost genetske delitve (diferenciacije) glede na vrednosti Fst je nihala glede na takson in je znašala 0,119 za Gallus domesticus; 0,043 za Equus caballus in 0,03 za Bos taurus. Genetska diferenciacija med populacijami, ocenjena z analizo molekularne variance (AMOVA), je bila pri različnih taksonih v razponu od 3 do 14 odstotkov. Ključne besede: mikrosateliti; raznolikost; polimorfizem; populacija; lokalne pasme; govedo; konj; piščane