The high rate of periodont diseases in children with disabilities, the duration and severity of the disease, which leads to tooth loss and a decrease in the quality of life, exclude this problem from the medical sphere, and an important social approach to the problem is also important. The prevalence of inflammatory diseases in parodont in children with disabilities, their susceptibility to development and complications, and inadequate treatment and preventive measures clarify the importance of this problem in modern dentistry

Eronov Yoqub Quvatovich

English

JOURNAL OF HEALTHCARE AND LIFE-SCIENCE RESEARCH

Journal of Healthcare and Life-Science Research Vol. 3, No. 6, 2024    ISSN: 2181-4368   22  BACTERIOLGIC ANALYSIS IN PАRODONTITIS IN CHILDREN WITH DISABILITIES    Annotation: The high rate of periodont diseases in children with disabilities, the duration and severity of the disease, which leads to tooth loss and a decrease in the quality of life, exclude this problem from the medical sphere, and an important social approach to the problem is also important. The prevalence of inflammatory diseases in parodont in children with disabilities, their susceptibility to development and complications, and inadequate treatment and preventive measures clarify the importance of this problem in modern dentistry. Keywords:  Information about the authors Eronov Yoqub Quvatovich Bukhara State Medical Institute   Relevance of the study: The high rate of periodont diseases in children with disabilities, the duration and severity of the disease, which leads to tooth loss and a decrease in the quality of life, exclude this problem from the medical sphere, and an important social approach to the problem is also important. The prevalence of inflammatory diseases in parodont in children with disabilities, their susceptibility to development and complications, and inadequate treatment and preventive measures clarify the importance of this problem in modern dentistry. To the health of the oral cavity and the whole organism of parodont diseases in children with limited capacity, chronic infection-inflammatory processes in the soft tissues of the parodont and, in parallel, severe and difficult to reverse catarrhal gingivitis – testifies to the extremely negative effect of the growth of risk factors leading to the transition to diffuse parodontitis. The problem of the treatment of periodont diseases in children with disabilities remains relevant to this day. The complex treatment of chronic diffuse periodontitis, a high level of this pathology, a tendency not to deviate from the growth of morbidity, despite certain achievements in the mucosalization of its prophylaxis, testifies to the relevance of attention to this problem. The following rules were followed when material was obtained for microbiological research: 1. No drugs were applied to children's mshq until the Material was obtained. 2. Children did not brush their teeth before the Material was obtained. 3. At each working stage, the receipt of the material was carried out at least 2 hours after the food was received. 4. The material obtained for the study was delivered to the bacteriological laboratory within 30 minutes of receipt. Journal of Healthcare and Life-Science Research Vol. 3, No. 6, 2024    ISSN: 2181-4368   23 The following material was taken for microbiological study in children of a patient with limited access to chronic diffuse periodontitis: -the contents of the tooth-milk vodka were taken into a sterile, hygroscopic vata fuse in the dental tube and also closed with a sterile lid, placing it in a sterile vial with a physiological mixture of 1 ml. The separation of microorganisms from their natural habitat was carried out by planting the studied materials in artificial nutrient environments. The method we are studying has the research name "cultural" [1.3.5.7.9]. The materials under study nutrient media were planted with 0.1 ml of oral fluid and a physiological mixture and 0.1 ml of a vial with the contents of the tooth-gum vodka. The material under study was planted in Petri cups primary to a dense nutrient medium. Taking the material into a pipette and slightly opening the cup, a drop was lowered into the medium and rubbed with a spatula over the entire surface of the agar. To separate the general microflora, it was planted in blood agar prepared in the following way. 5-10% of the Animal (Sheep, rabbit, large-horned mole) diffibrinated or completely freshly obtained blood or human blood waste was added to the dissolved and cooled nutrient agar up to 45-0oc degrees, and then the sterility was checked in advance, planted in sugar water, leaving it in a thermostat for 18-20 hours. Blood agar without forming a foam, is thoroughly mixed and poured into cups with a layer of 3-4 mm. Cultivation is carried out in a thermostat at a temperature of 37oC for 18-20 hours. The Endo environment was used to detect intestinal microflora. To prepare it, 100 ml of ordinary agar (pH 7.4) was heated in a water bath or steam Park, cooled to 70oc degrees and added 1 g of chemically pure lactose dissolved in a sterile test tube of pre-distilled and boiled water. In separate test tubes: 1) 2-3 ml of the alcohol-saturated mixture of the main Fuxin 2) 10 ml of the 10% sulfite sodium water mixture (Na2SO3). In a sterile test tube, 1 ml of fuchsin mixture was measured and a sulfite sodium mixture was added (light pink color) until the fuchsin discolored. The prepared mixture was poured into heated Agar, without forming a foam, mixed well and poured into cups with a layer of 3-4 mm. Warm agar has a light-pink color, when it cools down, it discolors. Grown in a thermostat at a temperature of 37oC for 18-20 hours. In the material under study, Sandida was planted in the Saburo environment to identify fungi of the species. The basis of this environment is the drojja water. 80 g of culinary drojja is taken (or 20 g of dry drojja) per 1 liter of water pipe water (not distilled), boiled for 15 minutes, filtered through a paper filter, poured into vials and 1 ATM.Sterilized for 2 minutes. 100 ml of sterilized, 1% pepton, 2% agar is added to drojja water, heated until dissolved, then 4% glucose is added (or maltose), filtered, poured into a test tube (pH 5.8) sterilized at 0.5 ATM for 20 minutes. After sterilization, the environment in the test tubes is released. Grown in a thermostat at a temperature of 37oC for 5 days [2.4.6.8.10.12.14.16.18.20]. The interpretation of the results of the microbiological study of parodont diseases in children with disabilities was carried out taking into account the differential symptoms that are characteristic of each type of bacteria, formed during the growth of the colony. Tilla for staphylococci (S.aureus) or white (S. epidermidis, S. Saprofhyticus) colonies are endemic. In micrococcus, colonies are usually colored yellow (with different shades – yellow-green to orange) or pink (red to Orange). S.many strains of aureus and S.some strains of epidermidis are dissolved by erythrocytes, forming a transparent area of hemolysis around the colonies. Micrococcus do not have hemolytic properties. Journal of Healthcare and Life-Science Research Vol. 3, No. 6, 2024    ISSN: 2181-4368   24 Streptococci are differentiated by the appearance of hemolysis in blood agar, which is associated with the lysis of cross-erythrocytes. At the same time, a transparent area is formed around the colonies with a complete covering of the environment from a tenth to a few millimeters wide. colonies of β-hemolytic streptococci may appear as follows: a mucoid 1.5-2.5 mm in diameter, in a straight circular shape, in the appearance of memorizing dew drops; Thunder-behold, 1.5-2.5 mm in diameter, round colonial, gray-white, with a slightly raised center; smooth, small,1-1.5 mm in diameter, spherical colony with smooth edges, bright wet surface, α-hemolytic or or bruised streptococci hemispherical in blood agar, Green in the area, forming small colonies 1-1. 5 mm in diameter,forming an α-reaction on a smooth or coarse gray-green surface; they do not have a blood agar appearance and are called nonhemolytic. Neisseries appear on the surface of blood agar in the form of smooth colonies with smooth circles around the edges, a bright or rough colony on the surface, curved edges, a strange superficial appearance with a wrong shape, some with yellow pigment. Moraxell's species grow in the form of a large hemispherical, circular, moist, sometimes mucous colony with or without a small area of hemolysis. Microbes of the asinetobaster type grow large, white, bright often mucous colony visigor, around which there is a possibility of a small hemolysis area. Corybebacterium colonies are circular, opaque, oily, small or large, creamy, white-yellow, orange-liver colored, smooth without a hemolysis area [11.13.15.17.19].  In an" Endo " environment, colonies of Representatives of the family Enterobacteriaceae are bulging, straight-circled, more or less opalesized, sometimes glabrous in appearance. They can be colorless, especially pink or gray in large colonies with a black tint with a more or less pronounced Center. Colonies of fungi of the Candida type are bulging, smooth, shiny, but not wet, smooth or slightly wrinkled, first white, then cream-colored. From a vial with a content of 0.1 ml of oral liquid and 1 ml of physiological mixture and tooth-milk vodka, colonies grown in nutrient media were calculated. The quantitative and qualitative analysis of the microflora of the tooth-gum Wasp helped to establish their norm in healthy children in the control group. It has been observed that the total number of anaerobic and facultative group germs of periodont diseases in children with limited capacity is largely the same. It should be mentioned that the leading place in the facultative group of microbes belongs to staphylococci and streptococci. The Epidermal Staphylococcus and Streptococcus salivarius stamps form the largest group among the grammusbat coccus group. Grammanphium is low in flora and was mainly manifested by the Esherich, Proteus and Klebsiella. With the development of chronic diffuse periodontitis in the oral cavity of periodont diseases in children with limited capacity, the ratio of microflora changes slightly. In the mild form of STP, there is a sufficiently reliable displacement of microorganisms in the facultative Group, a decrease in the amount of microflora of the anaerobic group. Str.against the background of a decrease in the amount of salivarius, str.mutans kokk Flora has been found to grow. Some types of negative flora have a tendency to grow. This is especially noticeable in microbes belonging to Proteus. This species characterizes the growth of this microflora, predicts the possible development of decay processes [1.2.3.4.5]. Significant changes in the quantitative and qualitative types of oral microflora were found in the middle and severe STP delay. A decrease in the amount of lactobacilli by almost 2 times was established, which testifies to a sharp shortage of anaerobic flora. Journal of Healthcare and Life-Science Research Vol. 3, No. 6, 2024    ISSN: 2181-4368   25 When STP was observed in children with limited capacity, it marked the growth of Escherichia, Proteus, and klibsiel strains in studies of the Gram-negative flora. In this group, it should be mentioned that the microflora of the Proteus has grown significantly, from 1.30±0.04 Koe/ml to 4.95±0.21 Koe/ml in the norm, from which the increase is occurring in order 3-4. Against this background, there was also an increase in fungi of the Sandida type, which is able to call the development of candidiasis stomatitis when it accumulates in large quantities [5.6.7.8]. Conclusion. Thus, the quantitative and qualitative changes detected in automicrophlora in the oral cavity in children with limited capacity with scattered periodontitis of surinkali can lead to prolonged course of the disease, frequent recurrence and chronicness of the process. Microorganisms that are permanently present in the oral cavity can include highly detectable Streptococcus species of aerobes as well as fungi of Candida species. The leading flora in the oral cavity of children when the tissues of the parodont are clinically healthy are streptococci. When children have SKG, however, all strains of Streptococci lose their leadership position, which is especially true of STR, which in moderation will be equal to 100%.noticeable on salivarius. With the development of STP in the oral cavity, there is also an increase in fungi of the Candida type, which leads to the sharp development of candidiasis stomatitis in the oral cavity of children.  Literature used 1. Эронов Ё. К. 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BACTERIOLGIC ANALYSIS IN PАRODONTITIS IN CHILDREN WITH DISABILITIES

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BACTERIOLGIC ANALYSIS IN PАRODONTITIS IN CHILDREN WITH DISABILITIES

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