Adriaensen, Dirk

Avula, Leela

Buckinx, Roeland

Cox, Eric

Favoreel, Herman

Stuyven, Edith

Timmermans, Jean-Pierre

Van Nassauw, Luc

English

Ghent University Academic Bibliography

INTESTINAL	  INFLAMMATION	  INDUCED	  CHANGES	  IN	  THE	  EXPRESSION	  AND	  DISTRIBUTION	  OF	  MAS-­‐RELATED	  GENE	  RECEPTORS	  IN	  THE	  MURINE	  ILEUM	  L.R.	  Avula	  1,	  L.	  Van	  Nassauw1,	  E.	  Stuyven2,	  R.	  Buckinx1,	  D.	  Adriaensen1,	  H.	  Favoreel2,	  E.	  Cox2,	  J-­‐P	  Timmermans	  1	  1	  Departmentof	  Veterinary	  Medicine,	  Laboratory	  of	  Cell	  Biology	  &	  Histology,	  University	  of	  Antwerp	  2	  Laboratory	  for	  Immunology,	  Fac	  of	  Vet	  Medicine,	  Ghent	  University	   INTRODUCTION/OBJECTIVES: Mas-related gene receptors (Mrgs) constitute a complex family of orphan GPCRs, some of which are predominantly expressed in spinal sensory neurons. Some Mrgs are suggested to participate in the regulation of inflammatory responses to IgE-independent mast cell activation, as well as in the neuroimmune communication.  AIMS & Methods: The lack of data concerning Mrgs in the gastrointestinal tract, in both healthy and inflamed conditions, led us to investigate the expression and putative function of Mrgs in the non-inflamed and Schistosoma mansoni-infected murine ileum. Immunohistochemical analyses were performed on cryosections and whole-mounts of non-inflamed and inflamed ileum using custom-made polyclonal antisera against MrgA4, MrgB2, MrgB8 and MrgB10, as well as a commercially available antiserum against MrgD. Real-Time PCR was performed on non-inflamed and inflamed ileum to detect and quantify the expression levels of MrgA4, MrgB2, MrgB8, MrgB10 and MrgD mRNAs.  RESULTS: In the non-inflamed ileum, immunohistochemistry revealed no MrgB10 or MrgD immunoreactivity (IR), whereas moderate MrgA4, MrgB2 and MrgB8 IR was detected in some neuronal somata in the enteric plexuses. In the inflamed ileum, MrgA4, MrgB2, MrgB8, MrgB10 and MrgD IR was observed in a significantly increased number of neuronal somata in both enteric plexuses (Table 1), as well as in nerve fibres in the tunica muscularis and the lamina propria.  Table 1. Proportional expression of Mrgs in enteric neurons in the non-inflamed and inflamed ileum. *= P-value<0.05    Non-inflamed ileum Inflamed ileum MrgA4 0.5% 7.0%* MrgB2 0.4% 4.0%* MrgB8 1.5% 12.0%* MrgB10 0.0% 6.0%* MrgD 0.0% 5.0%*  Colocalisation studies using antibodies against neurochemical markers demonstrated that MrgA4-, MrgB2-, MrgB8-, MrgB10- and MrgD-expressing neurons were predominantly intrinsic sensory neurons. In the inflamed ileum, MrgB10 and MrgD were also detected in mucosal mast cells (MMCs). These results were corroborated by Real-Time PCR which demonstrated the upregulation of MrgA4, MrgB2, MrgB8, MrgB10 and MrgD mRNAs in the inflamed ileum.  CONCLUSION: The increased expression of MrgA4, MrgB2, MrgB8, MrgB10 and MrgD in neurons and nerve fibres, as well as the presence of MrgB10 and MrgD in MMCs during inflammation indicate the involvement of Mrgs in neuronal and mast cell responses during intestinal inflammation. Future work should aim at elucidating the (patho)physiological role of these Mrgs. Supported by the FWO-grant G.0179.08N. 	  

Intestinal inflammation induced changes in the expression and distribution of Mas-related gene receptors in the murine ileum

https://biblio.ugent.be/publication/2007741/file/2007847.pdf

Intestinal inflammation induced changes in the expression and distribution of Mas-related gene receptors in the murine ileum

Abstract

Similar works

Full text

Available Versions

Ghent University Academic Bibliography