Cambré, Isabelle

Coudenys, Julie

De Muynck, Amélie

Elewaut, Dirk

Gaublomme, Djoere

Jacques, Peggy

Lambrecht, Stijn

Louagie, Els

Pauwels, Elin

Schryvers, Nadia

Van Hoorebeke, Luc

Vanderniepen, Pieter

Verheugen, Eveline

Windels, Fien

English

Ghent University Academic Bibliography

2nd UGCT seminar 10 September 2015  Application of µCT in arthritis and osteoporosis research.  Els Louagie1*, Isabelle Cambré1*, Amelie De Muynck2, Djoere Gaublomme1, Elin Pauwels2 , Pieter Vanderniepen2 , Fien Windels1 , Julie Coudenys1 , Nadia Schryvers1 , Eveline Verheugen1 , Stijn Lambrecht1 , Peggy Jacques1, Luc Van Hoorebeke2, Dirk Elewaut1 1Laboratory for Molecular Immunology and Inflammation, Department of Rheumatology, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185, 9000 Gent, Belgium     VIB Inflammation Research Center, Ghent, Belgium [email: Els.Louagie@UGent.be] 2UGCT, Department of Physics and Astronomy, Faculty of Sciences, Ghent University, Proeftuinstraat 86-N12, 9000 Gent, Belgium  [email: Amelie.Demuynck@UGent.be] *contributed equally  Keywords: Arthritis, Bone, Mouse, Osteoporosis  ABSTRACT  Arthritis and osteoporosis are chronic disorders in which bone is dramatically affected. Bone erosion in the joints is a disabling hallmark of Rheumatoid Arthritis (RA) and Spondyloarthritides (SpA), two prototypic forms of chronic arthritis in men. In addition, bone new formation is a typical feature of SpA which can lead to ankylosis in some cases. Osteoporosis, on the other hand is a non-inflammation dependent bone disorder and the most significant underlying cause of skeletal fractures in postmenopausal women.  Experimental mouse models mimicking different characteristics of those disorders are being used to understand their pathology and to test treatments. Since decades two-dimensional sections by histology have been the gold standard to assess bone damage in these diseases (Seeuws et al. 2010). Recently, three-dimensional images can be produced by the application of micro Computed Tomography. High resolution µCT has enabled us to visualise bone structure, bone erosion and bone new formation in great detail.   Samples were scanned at the Centre for X-Ray Tomography of the Ghent University (UGCT) on HECTOR (Masschaele et al. 2013). The resulting scan images have a voxel size of 4 micrometer. The obtained projection images were reconstructed using Octopus (Vlassenbroeck et al. 2007), a commercial software originally developed by UGCT. 3D visualisations were made using the commercial rendering software VGStudio_MAX. Bone parameters such as bone erosion, bone volume/total volume, cortical thickness, trabecular thickness and trabecular separation were calculated by an in house developed script using Image J. We have been able to use this in house script to accurately measure changes in bone parameters after ovariectomy in mice, mimicking postmenopausal osteoporosis. Evaluation of bone erosion in paws of mice after induction of arthritis and in conditions of increased or decreased biomechanical loading has highlighted a critical role for biomechanical stress in both models of RA and SpA (Jacques et al. 2014).   2nd UGCT seminar 10 September 2015         Figure 1: upper panel: 3D visualisation of microCT scans of knees of a mild arthritic (left) and a severe arthritic mouse (right). Lower panel: feet of control arthritic mouse (left) and arthritic mouse able to voluntary run in a running wheel (right), both feet had the same clinical inflammatory score.  REFERENCES  Jacques, P., Lambrecht, S., Verheugen, E., Pauwels, E., Kollias, G., Armaka, M., Verhoye, M., Van der Linden, A., Achten, R., Lories, R.J., Elewaut, D. (2014). "Proof of concept: enthesitis and new bone formation in spondyloarhtritis are driven by mechanical strain and stromal cells." Ann Rheum Dis. 73(2): 437-45.  Masschaele, B., Dierick, M., Van Loo, D., Boone, M.N., Brabant, L., Pauwels, E., Cnudde, V., Van Hoorebeke, L. (2013). "HECTOR: A 240kV micro-CT setup optimized for research." Journal of Physics: Conference Series. 463(1): 012012.  Seeuws, S., Jacques, P., Van Praet, J., Drennan, M., Coudenys, J., Decruy, T., Deschepper, E., Lepescheux, L., Pujuguet, P., Oste, L., Vandeghinste, N., Brys, R., Verbruggen, G., Elewaut, D. (2010). “A multiparameter approach to monitor disease activity in collagen-induced arthritis.” Arthritis Res Ther. 12(4): R160  Vlassenbroeck, M., Dierick, M., Masschaele, B., Cnudde, L., Van Hoorebeke, L., Jacobs, P. (2007). "Software tools for quantification of X-ray microtomography at the UGCT." Nuclear Instruments and Methods in Physics Research A 580 442-445. 

Application of µCT in arthritis and osteoporosis research

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Application of µCT in arthritis and osteoporosis research

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