Croubels, Siska

De Backer, Patrick

De Baere, Siegrid

De Saeger, Sarah

Eeckhout, Mia

Goossens, Joline

Osselaere, Ann

English

Ghent University Academic Bibliography

 Influence of mycotoxin detoxifying agents on the plasma pharmacokinetics and tissue residues of amoxicillin and oxytetracycline in poultry  Osselaere A.1, Goossens J. 1, De Baere S. 1, De Saeger S.2, Eeckhout M.3, De Backer P. 1, Croubels S. 1   1. Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University,  Salisburylaan 133, 9820 Merelbeke, Belgium. 2. Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Gent, Belgium. 3. Ghent University College, Schoonmeerstraat 52, 9000 Gent, Belgium.  email: ann.osselaere@ugent.be  Mycotoxin-detoxifying agents are frequently used feed additives for reduction of mycotoxicoses. Depending on their mode of action, the detoxifiers are divided in two different classes: adsorbing and biotransforming agents. The first ones are able to bind mycotoxins, the second ones have the ability to degrade mycotoxins.  In poultry, amoxicillin and oxytetracycline are frequently administrated in the drinking water for the treatment of bacterial diseases. Mycotoxin-detoxifying agents possibly could interact with the pharmacokinetics of these antibiotics. To confirm this hypothesis, following experiment was conducted. Forty-eight 1-day-old Ross chicks were brooded under standard husbandry conditions. After one week acclimatization the animals were divided into six groups, each group consisting of eight animals. The chickens were fed diets containing no detoxifying agent (group 1 and 2), an adsorbing agent (group 3 and 4) or a biotransforming agent (group 5 and 6).  After three weeks of feeding, a kinetic study of oxytetracycline was performed in group 1, 3 and 5 (Table 1).  Table 1. Timetable of the critical events of the animal experiments with oxytetracycline  A similar experiment was performed for amoxicillin in group 2, 4 and 6 (Table 2).  Table 2. Timetable of the critical events of the animal experiments with amoxicillin  The concentration of both antibiotics in plasma and tissues was determined by validated LC-MS/MS methods. Plasma pharmacokinetic parameters (AUC0->∞,  AUC0->t, Cmax, Tmax,T1/2) were calculated using WinNonlin. The results of this experiment will be presented at the conference. Acknowledgements: The study was supported by the BOF-project N0 01J08309 from Ghent University. The assistance of J. Lambrecht en A. Van den Bussche is gratefully appreciated.  References EFSA, 2009. Review of mycotoxin-detoxifying agents used as feed additives: mode of action, efficacy and feed/food safety. The European Food Standard Agency (EFSA).  Day in the study D28 D29-33 D34 D35 8 a.m. Oral bolus oxytetracycline    (100 mg/kg B.W.), followed by a kinetic study during 24 hours Oral bolus oxytetracycline       (50 mg/kg B.W.)  Oxytetracycline in water (100 mg  oxytetracycline/kg B.W.)  Oral bolus  oxytetracycline      (50 mg/kg B.W.)  Refreshing of the medicated water (100 mg  oxytetracycline/kg B.W.)  Euthanasia of the animals  16 hours after withdrawal of medicated water and collection of liver and kidneys 8 p.m.  Refreshing of the medicated water (100 mg  oxytetracycline/kg B.W.) Unmedicated water Day in the study D28 D29-31 D32 D33 8 a.m. Oral bolus amoxicillin           (20 mg/kg B.W.), followed by a kinetic study during 10 hours Oral bolus amoxicillin           (10 mg/kg B.W.)  Refreshing of the medicated water (20 mg  amoxicillin/kg B.W.) Oral bolus amoxicillin            (10 mg/kg B.W.)  Refreshing of the medicated water (20 mg  amoxicillin/kg B.W.)  Euthanasia of the animals  12 hours after withdrawal of  medicated water and collection of liver and kidneys 8 p.m. 20 mg amoxicillin/kg B.W. added in the water Refreshing of the medicated water (20 mg  amoxicillin/kg B.W.) Unmedicated water 

Influence of mycotoxin detoxifying agents on the plasma pharmacokinetics and tissue residues of amoxicillin and oxytetracycline in poultry

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Influence of mycotoxin detoxifying agents on the plasma pharmacokinetics and tissue residues of amoxicillin and oxytetracycline in poultry

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Ghent University Academic Bibliography